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Yorodumi- PDB-1g4d: NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1g4d | ||||||
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Title | NMR STRUCTURE OF THE MU BACTERIOPHAGE REPRESSOR DNA-BINDING DOMAIN/DNA COMPLEX | ||||||
Components |
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Keywords | Viral protein/DNA / protein-DNA complex / helix-turn-helix / winged-helix / bacteriophage Mu / repressor / virus/viral protein / Viral protein-DNA COMPLEX | ||||||
Function / homology | Function and homology information viral latency / latency-replication decision / transcription repressor complex / host cell cytoplasm / DNA binding Similarity search - Function | ||||||
Biological species | Enterobacteria phage Mu (virus) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wojciak, J.M. / Iwahara, J. / Clubb, R.T. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2001 Title: The Mu repressor-DNA complex contains an immobilized 'wing' within the minor groove. Authors: Wojciak, J.M. / Iwahara, J. / Clubb, R.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1g4d.cif.gz | 1021.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1g4d.ent.gz | 849.6 KB | Display | PDB format |
PDBx/mmJSON format | 1g4d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1g4d_validation.pdf.gz | 375.6 KB | Display | wwPDB validaton report |
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Full document | 1g4d_full_validation.pdf.gz | 835.5 KB | Display | |
Data in XML | 1g4d_validation.xml.gz | 44 KB | Display | |
Data in CIF | 1g4d_validation.cif.gz | 77.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g4/1g4d ftp://data.pdbj.org/pub/pdb/validation_reports/g4/1g4d | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 4839.151 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 4955.256 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 7543.704 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DNA-BINDING DOMAIN (RESIDUES 13-81) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage Mu (virus) / Genus: Mu-like viruses / Gene: MU C / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P06019 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||
NMR representative | Selection criteria: lowest energy | ||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers submitted total number: 25 |