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Open data
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Basic information
Entry | Database: PDB / ID: 1g01 | ||||||
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Title | ALKALINE CELLULASE K CATALYTIC DOMAIN | ||||||
![]() | ENDOGLUCANASE | ||||||
![]() | HYDROLASE / Alpha/beta barrel / TIM barrel | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Shirai, T. / Ishida, H. / Noda, J. / Yamane, T. / Ozaki, K. / Hakamada, Y. / Ito, S. | ||||||
![]() | ![]() Title: Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme. Authors: Shirai, T. / Ishida, H. / Noda, J. / Yamane, T. / Ozaki, K. / Hakamada, Y. / Ito, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97 KB | Display | ![]() |
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PDB format | ![]() | 72.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 378.4 KB | Display | ![]() |
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Full document | ![]() | 386.2 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 16.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 40367.254 Da / Num. of mol.: 1 / Fragment: ALKALINE CELLULASE K CATALYTIC DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-CD / #3: Chemical | ChemComp-ACY / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.2 Å3/Da / Density % sol: 70.74 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: cadmium sulfate hydrate, sodium acetate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Feb 1, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→8 Å / Num. all: 54119 / Num. obs: 49176 / % possible obs: 90.9 % / Observed criterion σ(I): 1 / Redundancy: 11.7 % / Rmerge(I) obs: 0.077 / Net I/σ(I): 20.1 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.202 / % possible all: 83.8 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 40 Å |
Reflection shell | *PLUS % possible obs: 83.8 % / Num. unique obs: 4496 / Mean I/σ(I) obs: 5.9 |
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Processing
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Refinement | Resolution: 1.9→8 Å / σ(F): 3 / Stereochemistry target values: Engh & HUber
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Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 8 Å / σ(F): 3 / % reflection Rfree: 5 % / Rfactor obs: 0.199 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 1.97 Å / Rfactor Rfree: 0.274 / Num. reflection Rfree: 214 / % reflection Rfree: 5 % / Num. reflection obs: 4493 / Rfactor all: 0.264 |