+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1fub | |||||||||
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タイトル | FIRST PROTEIN STRUCTURE DETERMINED FROM X-RAY POWDER DIFFRACTION DATA | |||||||||
要素 |
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キーワード | HORMONE/GROWTH FACTOR / POWDER DIFFRACTION / RIETVELD REFINEMENT / INSULIN / HORMONE-GROWTH FACTOR COMPLEX | |||||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / negative regulation of acute inflammatory response / Regulation of gene expression in beta cells / alpha-beta T cell activation / regulation of amino acid metabolic process / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / regulation of protein localization to plasma membrane / fatty acid homeostasis / negative regulation of gluconeogenesis / negative regulation of lipid catabolic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / positive regulation of insulin receptor signaling pathway / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / nitric oxide-cGMP-mediated signaling / positive regulation of protein autophosphorylation / insulin-like growth factor receptor binding / transport vesicle / Insulin receptor recycling / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / NPAS4 regulates expression of target genes / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / positive regulation of nitric-oxide synthase activity / acute-phase response / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / cognition / Regulation of insulin secretion / endosome lumen / positive regulation of D-glucose import / positive regulation of protein secretion / negative regulation of proteolysis / positive regulation of cell differentiation / regulation of transmembrane transporter activity / insulin receptor binding / wound healing / negative regulation of protein catabolic process / regulation of synaptic plasticity / hormone activity / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / vasodilation / glucose metabolic process / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / glucose homeostasis / regulation of protein localization / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / protease binding / secretory granule lumen / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / regulation of DNA-templated transcription / positive regulation of gene expression / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | |||||||||
手法 | 粉末回折 / シンクロトロン | |||||||||
データ登録者 | Von Dreele, R.B. / Stephens, P.W. / Blessing, R.H. / Smith, G.D. | |||||||||
引用 | ジャーナル: Acta Crystallogr.,Sect.D / 年: 2000 タイトル: The first protein crystal structure determined from high-resolution X-ray powder diffraction data: a variant of T3R3 human insulin-zinc complex produced by grinding. 著者: Von Dreele, R.B. / Stephens, P.W. / Smith, G.D. / Blessing, R.H. #1: ジャーナル: J.Appl.Crystallogr. / 年: 1999 タイトル: Combined Rietveld and Stereochemical Restraint Refinement of a Protein Crystal Structure 著者: Von Dreele, R.B. #2: ジャーナル: Biochemistry / 年: 1994 タイトル: Crystallographic Evidence for Dual Coordinate Aroun in the T3R3 Human Insulin Hexamer 著者: Smith, G.D. / Ciszak, E. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1fub.cif.gz | 24.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1fub.ent.gz | 17.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1fub.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1fub_validation.pdf.gz | 360.4 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1fub_full_validation.pdf.gz | 373.1 KB | 表示 | |
XML形式データ | 1fub_validation.xml.gz | 5.4 KB | 表示 | |
CIF形式データ | 1fub_validation.cif.gz | 7.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/fu/1fub ftp://data.pdbj.org/pub/pdb/validation_reports/fu/1fub | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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2 |
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Components on special symmetry positions |
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-要素
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 2 / 断片: A CHAIN OF T3R3 VARIANT / 由来タイプ: 合成 / 詳細: This sequence occurs naturally in humans / 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 断片: B CHAIN OF T3R3 VARIANT / 由来タイプ: 合成 / 詳細: This sequence occurs naturally in humans / 参照: UniProt: P01308 #3: 化合物 | #4: 化合物 | #5: 化合物 | ChemComp-NA / | |
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-実験情報
-実験
実験 | 手法: 粉末回折 |
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-試料調製
結晶 | マシュー密度: 2.04 Å3/Da / 溶媒含有率: 39.64 % |
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結晶化 | 温度: 300 K / 手法: grinding / 詳細: grinding, temperature 300K |
結晶化 | *PLUS 手法: unknown / 詳細: ground into powder |
溶液の組成 | *PLUS 濃度: 1.0 M / 化学式: NaCl |
-データ収集
回折 | 平均測定温度: 300 K |
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放射光源 | 由来: シンクロトロン / サイト: NSLS / ビームライン: X3B1 / 波長: 0.700233 |
検出器 | タイプ: OTHER / 検出器: DIFFRACTOMETER / 日付: 1999年10月21日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.700233 Å / 相対比: 1 |