+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1ftn | ||||||
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タイトル | CRYSTAL STRUCTURE OF THE HUMAN RHOA/GDP COMPLEX | ||||||
要素 | TRANSFORMING PROTEIN RHOA (H12) | ||||||
キーワード | PROTO-ONCOGENE / GTP-BINDING / PRENYLATION / LIPOPROTEIN / SMALL P-PROTEIN | ||||||
機能・相同性 | 機能・相同性情報 alpha-beta T cell lineage commitment / aortic valve formation / mitotic cleavage furrow formation / positive regulation of lipase activity / bone trabecula morphogenesis / endothelial tube lumen extension / skeletal muscle satellite cell migration / positive regulation of vascular associated smooth muscle contraction / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / regulation of osteoblast proliferation ...alpha-beta T cell lineage commitment / aortic valve formation / mitotic cleavage furrow formation / positive regulation of lipase activity / bone trabecula morphogenesis / endothelial tube lumen extension / skeletal muscle satellite cell migration / positive regulation of vascular associated smooth muscle contraction / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / regulation of osteoblast proliferation / SLIT2:ROBO1 increases RHOA activity / RHO GTPases Activate Rhotekin and Rhophilins / Roundabout signaling pathway / negative regulation of intracellular steroid hormone receptor signaling pathway / Axonal growth inhibition (RHOA activation) / Axonal growth stimulation / regulation of neural precursor cell proliferation / cleavage furrow formation / regulation of modification of postsynaptic actin cytoskeleton / forebrain radial glial cell differentiation / cell junction assembly / apical junction assembly / cellular response to chemokine / negative regulation of cell migration involved in sprouting angiogenesis / regulation of systemic arterial blood pressure by endothelin / beta selection / establishment of epithelial cell apical/basal polarity / negative regulation of cell size / RHO GTPases Activate ROCKs / regulation of modification of postsynaptic structure / negative regulation of oxidative phosphorylation / negative regulation of motor neuron apoptotic process / RHO GTPases activate CIT / Sema4D induced cell migration and growth-cone collapse / RHO GTPases activate KTN1 / odontogenesis / PCP/CE pathway / apolipoprotein A-I-mediated signaling pathway / positive regulation of podosome assembly / negative regulation of cell-substrate adhesion / positive regulation of alpha-beta T cell differentiation / positive regulation of leukocyte adhesion to vascular endothelial cell / Sema4D mediated inhibition of cell attachment and migration / motor neuron apoptotic process / Wnt signaling pathway, planar cell polarity pathway / PI3K/AKT activation / wound healing, spreading of cells / apical junction complex / ossification involved in bone maturation / regulation of focal adhesion assembly / negative chemotaxis / myosin binding / EPHA-mediated growth cone collapse / stress fiber assembly / positive regulation of cytokinesis / regulation of neuron projection development / RHOC GTPase cycle / cellular response to cytokine stimulus / androgen receptor signaling pathway / cerebral cortex cell migration / ERBB2 Regulates Cell Motility / cleavage furrow / Rho protein signal transduction / semaphorin-plexin signaling pathway / ficolin-1-rich granule membrane / mitotic spindle assembly / endothelial cell migration / RHOA GTPase cycle / positive regulation of T cell migration / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / regulation of microtubule cytoskeleton organization / cytoplasmic microtubule organization / negative regulation of reactive oxygen species biosynthetic process / RHO GTPases activate PKNs / skeletal muscle tissue development / GPVI-mediated activation cascade / positive regulation of stress fiber assembly / EPHB-mediated forward signaling / substantia nigra development / positive regulation of neuron differentiation / regulation of cell migration / cell-matrix adhesion / substrate adhesion-dependent cell spreading / secretory granule membrane / small monomeric GTPase / kidney development / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / cell periphery / regulation of actin cytoskeleton organization / RHO GTPases Activate Formins / neuron migration / cell morphogenesis / positive regulation of protein serine/threonine kinase activity / G protein activity / VEGFA-VEGFR2 Pathway / ruffle membrane / cytoplasmic side of plasma membrane / positive regulation of non-canonical NF-kappaB signal transduction / G beta:gamma signalling through PI3Kgamma / G alpha (12/13) signalling events 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 多重同系置換, 分子置換 / 解像度: 2.1 Å | ||||||
データ登録者 | Wei, Y. / Zhang, Y. / Derewenda, U. / Liu, X. / Minor, W. / Nakamoto, R.K. / Somlyo, A.V. / Somlyo, A.P. / Derewenda, Z.S. | ||||||
引用 | ジャーナル: Nat.Struct.Biol. / 年: 1997 タイトル: Crystal structure of RhoA-GDP and its functional implications. 著者: Wei, Y. / Zhang, Y. / Derewenda, U. / Liu, X. / Minor, W. / Nakamoto, R.K. / Somlyo, A.V. / Somlyo, A.P. / Derewenda, Z.S. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1ftn.cif.gz | 56.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1ftn.ent.gz | 38.9 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1ftn.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1ftn_validation.pdf.gz | 453.5 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1ftn_full_validation.pdf.gz | 462.1 KB | 表示 | |
XML形式データ | 1ftn_validation.xml.gz | 7.1 KB | 表示 | |
CIF形式データ | 1ftn_validation.cif.gz | 10.9 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ft/1ftn ftp://data.pdbj.org/pub/pdb/validation_reports/ft/1ftn | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 21766.088 Da / 分子数: 1 / 変異: F25N / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: SYNTHETIC HUMAN RHOA GENE / 遺伝子 (発現宿主): SYNTHETIC HUMAN RHOA GENE / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P61586 |
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#2: 化合物 | ChemComp-MG / |
#3: 化合物 | ChemComp-GDP / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.12 Å3/Da / 溶媒含有率: 42 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 7.5 / 詳細: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: EMBL/DESY, HAMBURG / ビームライン: X31 / 波長: 0.98 |
検出器 | タイプ: MAR scanner 180 mm plate / 検出器: IMAGE PLATE / 日付: 1996年12月4日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.98 Å / 相対比: 1 |
反射 | 解像度: 2.1→8 Å / Num. obs: 10913 / % possible obs: 97.3 % / 冗長度: 3.7 % / Rmerge(I) obs: 0.045 / Rsym value: 0.045 / Net I/σ(I): 14.8 |
反射 シェル | 解像度: 2.08→2.15 Å / Rmerge(I) obs: 0.121 / Mean I/σ(I) obs: 10.1 / % possible all: 81.1 |
反射 | *PLUS Num. measured all: 40036 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 多重同系置換, 分子置換 開始モデル: POLY ALANINE MODEL OF RAC1 解像度: 2.1→8 Å / σ(F): 0 /
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精密化ステップ | サイクル: LAST / 解像度: 2.1→8 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: REFMAC / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.186 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |