+Open data
-Basic information
Entry | Database: PDB / ID: 1fsl | ||||||
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Title | FERRIC SOYBEAN LEGHEMOGLOBIN COMPLEXED WITH NICOTINATE | ||||||
Components | LEGHEMOGLOBIN A | ||||||
Keywords | OXYGEN TRANSPORT / HEME / RESPIRATORY PROTEIN / NITROGEN FIXATION / MULTIGENE FAMILY | ||||||
Function / homology | Function and homology information response to nitrogen compound / nodulation / response to hydrogen sulfide / response to cobalt ion / oxygen carrier activity / oxygen binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Glycine max (soybean) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.3 Å | ||||||
Authors | Ellis, P.J. / Guss, J.M. / Freeman, H.C. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1997 Title: Structure of ferric soybean leghemoglobin a nicotinate at 2.3 A resolution. Authors: Ellis, P.J. / Appleby, C.A. / Guss, J.M. / Hunter, W.N. / Ollis, D.L. / Freeman, H.C. #1: Journal: Aust.J.Chem. / Year: 1983 Title: Crystal Structure of Soybean Ferric Leghaemoglobin a Nicotinate at a Resolution of 3.3 Angstrom Authors: Ollis, D.L. / Appleby, C.A. / Colman, P.M. / Cutten, A.E. / Guss, J.M. / Venkatappa, M.P. / Freeman, H.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fsl.cif.gz | 117.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fsl.ent.gz | 94.5 KB | Display | PDB format |
PDBx/mmJSON format | 1fsl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fsl_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 1fsl_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 1fsl_validation.xml.gz | 14.6 KB | Display | |
Data in CIF | 1fsl_validation.cif.gz | 19.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fs/1fsl ftp://data.pdbj.org/pub/pdb/validation_reports/fs/1fsl | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.461976, 0.030242, 0.886377), Vector: |
-Components
#1: Protein | Mass: 15260.336 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: FE(III), PH 6.0 / Source: (natural) Glycine max (soybean) / Organ: ROOT NODULE / References: UniProt: P02238 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 6 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.62 % |
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Crystal grow | pH: 6 / Details: pH 6.0 |
Crystal grow | *PLUS Method: other / Details: Ollis, D.L., (1983) Aust. J. Chem., 36, 451. |
-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: SEALED TUBE / Type: OTHER / Wavelength: 1.5418 |
Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: 1981 / Details: COLLIMATOR |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→8 Å / Num. obs: 14153 / Observed criterion σ(I): 0 / Redundancy: 1.3 % / Rmerge(I) obs: 0.061 |
Reflection | *PLUS Num. measured all: 18416 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.3→6 Å / σ(F): 2
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Displacement parameters | Biso mean: 12.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→6 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.158 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |