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Yorodumi- PDB-1fnb: REFINED CRYSTAL STRUCTURE OF SPINACH FERREDOXIN REDUCTASE AT 1.7 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fnb | |||||||||
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Title | REFINED CRYSTAL STRUCTURE OF SPINACH FERREDOXIN REDUCTASE AT 1.7 ANGSTROMS RESOLUTION: OXIDIZED, REDUCED, AND 2'-PHOSPHO-5'-AMP BOUND STATES | |||||||||
Components | FERREDOXIN-NADP+ REDUCTASE | |||||||||
Keywords | OXIDOREDUCTASE (NADP+(A) / FERREDOXIN(A)) | |||||||||
Function / homology | Function and homology information chloroplast thylakoid membrane protein complex / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / photosynthesis / electron transport chain Similarity search - Function | |||||||||
Biological species | Spinacia oleracea (spinach) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.7 Å | |||||||||
Authors | Bruns, C.M. / Karplus, P.A. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1995 Title: Refined crystal structure of spinach ferredoxin reductase at 1.7 A resolution: oxidized, reduced and 2'-phospho-5'-AMP bound states. Authors: Bruns, C.M. / Karplus, P.A. #1: Journal: Science / Year: 1991 Title: Atomic Structure of Ferredoxin-Nadp+ Reductase: Prototype for a Structurally Novel Flavoenzyme Family Authors: Karplus, P.A. / Daniels, M.J. / Herriott, J.R. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fnb.cif.gz | 78.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fnb.ent.gz | 57.8 KB | Display | PDB format |
PDBx/mmJSON format | 1fnb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fnb_validation.pdf.gz | 777.7 KB | Display | wwPDB validaton report |
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Full document | 1fnb_full_validation.pdf.gz | 787.1 KB | Display | |
Data in XML | 1fnb_validation.xml.gz | 16.6 KB | Display | |
Data in CIF | 1fnb_validation.cif.gz | 24 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fn/1fnb ftp://data.pdbj.org/pub/pdb/validation_reports/fn/1fnb | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 150 | ||||||||
Components on special symmetry positions |
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-Components
#1: Protein | Mass: 35381.699 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Spinacia oleracea (spinach) / References: UniProt: P00455, ferredoxin-NADP+ reductase |
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#2: Chemical | ChemComp-PO4 / |
#3: Chemical | ChemComp-SO4 / |
#4: Chemical | ChemComp-FAD / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.38 % | ||||||||||||||||||
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Crystal grow | Details: PH 4.6 | ||||||||||||||||||
Crystal | *PLUS Density % sol: 49 % | ||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 4.9 / Method: vapor diffusion, hanging drop | ||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 1.7 Å / Num. obs: 38542 / % possible obs: 98 % / Observed criterion σ(I): 3 / Redundancy: 2.5 % / Num. measured all: 145352 / Rmerge(I) obs: 0.05 |
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-Processing
Software | Name: TNT / Version: 5A / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Highest resolution: 1.7 Å / σ(I): 3 Details: PHASES PROVIDED FOR THE NATIVE STRUCTURE FACTORS ARE THOSE DETERMINED BY MIR WHEN AVAILABLE. PHASES ARE CALCULATED FOR THE REST. CALCULATED PHASES HAVE FIGURE-OF-MERIT REPORTED AS ZERO. ...Details: PHASES PROVIDED FOR THE NATIVE STRUCTURE FACTORS ARE THOSE DETERMINED BY MIR WHEN AVAILABLE. PHASES ARE CALCULATED FOR THE REST. CALCULATED PHASES HAVE FIGURE-OF-MERIT REPORTED AS ZERO. DURING REFINEMENT, THE MAXIMUM TEMPERATURE FACTOR ALLOWED WAS 100.00.
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Refinement step | Cycle: LAST / Highest resolution: 1.7 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 9999 Å | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 28 Å2 |