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Yorodumi- PDB-1fn4: CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fn4 | ||||||
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Title | CRYSTAL STRUCTURE OF FAB198, AN EFFICIENT PROTECTOR OF ACETYLCHOLINE RECEPTOR AGAINST MYASTHENOGENIC ANTIBODIES | ||||||
Components | (MONOCLONAL ANTIBODY AGAINST ACETYLCHOLINE RECEPTOR) x 2 | ||||||
Keywords | IMMUNE SYSTEM / FAB198 | ||||||
Function / homology | Function and homology information immunoglobulin complex, circulating / immunoglobulin receptor binding / complement activation, classical pathway / antigen binding / antibacterial humoral response Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.8 Å | ||||||
Authors | Poulas, K. / Eliopoulos, E. / Vatzaki, E. / Navaza, J. / Kontou, M. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2001 Title: Crystal structure of Fab198, an efficient protector of the acetylcholine receptor against myasthenogenic antibodies. Authors: Poulas, K. / Eliopoulos, E. / Vatzaki, E. / Navaza, J. / Kontou, M. / Oikonomakos, N. / Acharya, K.R. / Tzartos, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fn4.cif.gz | 174.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fn4.ent.gz | 143.7 KB | Display | PDB format |
PDBx/mmJSON format | 1fn4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fn/1fn4 ftp://data.pdbj.org/pub/pdb/validation_reports/fn/1fn4 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 23177.717 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Rattus norvegicus (Norway rat) / References: UniProt: P01835*PLUS #2: Protein | Mass: 23653.658 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Rattus norvegicus (Norway rat) / References: UniProt: P20760*PLUS #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.51 Å3/Da / Density % sol: 47.5 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 7.5 Details: 12 MG/ML FAB198, 1.6 M AS, 0.4 M CACL2, 0.1 M PB, 0.1% PEG 10000, PH 7.5, VAPOR DIFFUSION, SITTING DROP | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop / pH: 8.1 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ELETTRA / Beamline: 5.2R / Wavelength: 1 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. obs: 32382 / % possible obs: 98 % / Observed criterion σ(I): 2 / Redundancy: 4 % / Rmerge(I) obs: 0.046 |
Reflection shell | Resolution: 2.8→2.9 Å / Rmerge(I) obs: 0.217 / % possible all: 98.9 |
Reflection | *PLUS Num. measured all: 112032 |
Reflection shell | *PLUS % possible obs: 98.9 % |
-Processing
Software |
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Refinement | Resolution: 2.8→20 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0.001 / Cross valid method: THROUGHOUT / σ(F): 2 Details: ANOMALOUS PEPTIDE BOND DISTANCES CORRESPOND TO REGIONS POORLY DEFINED BY ELECTRON DENSITY
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Displacement parameters |
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.93 Å / Total num. of bins used: 6
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 2 / Num. reflection Rfree: 1619 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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