+Open data
-Basic information
Entry | Database: PDB / ID: 1faw | ||||||
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Title | GRAYLAG GOOSE HEMOGLOBIN (OXY FORM) | ||||||
Components |
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Keywords | OXYGEN STORAGE/TRANSPORT / OXYGEN TRANSPORT / HEME / RESPIRATORY PROTEIN / ERYTHROCYTE / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | Function and homology information hemoglobin complex / oxygen carrier activity / oxygen binding / iron ion binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Anser anser (Domestic goose) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3.09 Å | ||||||
Authors | Liang, Y.-H. / Liu, X.-Z. / Liu, S.-H. / Lu, G.-Y. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2001 Title: The structure of greylag goose oxy haemoglobin: the roles of four mutations compared with bar-headed goose haemoglobin. Authors: Liang, Y.H. / Liu, X.Z. / Liu, S.H. / Lu, G.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1faw.cif.gz | 114.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1faw.ent.gz | 91.7 KB | Display | PDB format |
PDBx/mmJSON format | 1faw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fa/1faw ftp://data.pdbj.org/pub/pdb/validation_reports/fa/1faw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15329.636 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Anser anser (Domestic goose) / Cell: ERYTHROCYTES / Cellular location: CYTOPLASM / Tissue: BLOOD / References: UniProt: P01989 #2: Protein | Mass: 16327.894 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Anser anser (Domestic goose) / Cell: ERYTHROCYTES / Cellular location: CYTOPLASM / Tissue: BLOOD / References: UniProt: P02117 #3: Chemical | ChemComp-HEM / #4: Chemical | ChemComp-OXY / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.56 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.8 Details: PEG 6000, potassium phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 291 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. all: 13099 / Num. obs: 12746 / % possible obs: 97.3 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4.4 % / Biso Wilson estimate: 37.2 Å2 / Rmerge(I) obs: 0.113 / Net I/σ(I): 7 |
Reflection shell | Resolution: 3→3.07 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.334 / Num. unique all: 834 / % possible all: 98 |
Reflection | *PLUS Highest resolution: 3.09 Å / % possible obs: 96.4 % / Num. measured all: 56170 |
Reflection shell | *PLUS Highest resolution: 3.1 Å / Lowest resolution: 3.2 Å / % possible obs: 95.2 % / Redundancy: 2.7 % |
-Processing
Software |
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Refinement | Resolution: 3.09→31.3 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 1092327.87 / Data cutoff low absF: 0 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 28.82 Å2 / ksol: 0.31 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.09→31.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.09→3.28 Å / Rfactor Rfree error: 0.022 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 8.9 % / Rfactor obs: 0.174 / Rfactor Rfree: 0.219 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 30.9 Å2 | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.303 / Rfactor Rwork: 0.266 |