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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1fav | |||||||||
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タイトル | THE STRUCTURE OF AN HIV-1 SPECIFIC CELL ENTRY INHIBITOR IN COMPLEX WITH THE HIV-1 GP41 TRIMERIC CORE | |||||||||
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![]() | VIRAL PROTEIN / HIV-1 / gp41 / inhibitor | |||||||||
機能・相同性 | ![]() FCERI mediated MAPK activation / Synthesis and processing of ENV and VPU / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / symbiont-mediated evasion of host immune response / mediator complex binding / positive regulation of establishment of T cell polarity ...FCERI mediated MAPK activation / Synthesis and processing of ENV and VPU / protein localization to nuclear periphery / Activation of the AP-1 family of transcription factors / response to amino acid starvation / negative regulation of ribosomal protein gene transcription by RNA polymerase II / positive regulation of cellular response to amino acid starvation / symbiont-mediated evasion of host immune response / mediator complex binding / positive regulation of establishment of T cell polarity / Alpha-defensins / Oxidative Stress Induced Senescence / Dectin-2 family / TFIID-class transcription factor complex binding / amino acid biosynthetic process / positive regulation of RNA polymerase II transcription preinitiation complex assembly / Binding and entry of HIV virion / positive regulation of transcription initiation by RNA polymerase II / cellular response to nutrient levels / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / cellular response to amino acid starvation / host cell endosome membrane / actin filament organization / Assembly Of The HIV Virion / Budding and maturation of HIV virion / RNA polymerase II transcription regulator complex / DNA-binding transcription activator activity, RNA polymerase II-specific / clathrin-dependent endocytosis of virus by host cell / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / viral protein processing / intracellular signal transduction / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / chromatin binding / virion attachment to host cell / host cell plasma membrane / virion membrane / structural molecule activity / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / identical protein binding / nucleus / membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | ![]() | |||||||||
![]() | Zhou, G. / Ferrer, M. / Chopra, R. / Strassmaier, T. / Weissenhorn, W. / Skehel, J.J. / Oprian, D. / Schreiber, S.L. / Harrison, S.C. / Wiley, D.C. | |||||||||
![]() | ![]() タイトル: The structure of an HIV-1 specific cell entry inhibitor in complex with the HIV-1 gp41 trimeric core. 著者: Zhou, G. / Ferrer, M. / Chopra, R. / Kapoor, T.M. / Strassmaier, T. / Weissenhorn, W. / Skehel, J.J. / Oprian, D. / Schreiber, S.L. / Harrison, S.C. / Wiley, D.C. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 35.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 24.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 436.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 438 KB | 表示 | |
XML形式データ | ![]() | 7.1 KB | 表示 | |
CIF形式データ | ![]() | 8.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 9268.784 Da / 分子数: 1 / 変異: L2I, V6I, L9I, N13I, L16I, V20I, L23I, V27I / 由来タイプ: 組換発現 詳細: CHIMERA CONSISTS OF A FRAGMENT OF GCN4 ZIPPER CLONED N-TERMINAL TO A FRAGMENT OF GP41 由来: (組換発現) ![]() ![]() ![]() ![]() 属: Lentivirus / プラスミド: PRSET / 遺伝子: GCN4,AAS3,ARG9,YEL009C / 発現宿主: ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 4049.338 Da / 分子数: 1 / Fragment: OUTER PEPTIDE OF GP41 OF HIV-1 / 由来タイプ: 合成 詳細: The peptide was chemically synthesized. The sequence of the peptide is naturally found in HIV-1. 参照: UniProt: P03377, UniProt: P04578*PLUS |
Has protein modification | Y |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.29 Å3/Da / 溶媒含有率: 46.29 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.9 詳細: 25mM PIPES, 0.25M MgAc, 2.5% isopropanol, 10mM Hepes, 37.5mM NaCl, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | |||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 詳細: drop consists of equal amounts of protein and reservoir solutionspH: 8.3 | |||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1998年9月14日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.54 Å / 相対比: 1 |
反射 | 解像度: 3→15 Å / Num. all: 2806 / Num. obs: 2574 / % possible obs: 88 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.072 |
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解析
ソフトウェア |
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精密化 | 解像度: 3→15 Å / σ(F): 2 / σ(I): 2 / 立体化学のターゲット値: Engh & Huber 詳細: Residue 125 chain C was modelled based on residues 124 and 126. The occupancy of this residue is 0.00.
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精密化ステップ | サイクル: LAST / 解像度: 3→15 Å
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拘束条件 |
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