+Open data
-Basic information
Entry | Database: PDB / ID: 1f9x | ||||||
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Title | AVERAGE NMR SOLUTION STRUCTURE OF THE BIR-3 DOMAIN OF XIAP | ||||||
Components | INHIBITOR OF APOPTOSIS PROTEIN XIAP | ||||||
Keywords | apoptosis inhibitor / Bir3 domain / inhibitor of apoptosis protein XIAP / Zinc finger / caspase-9 inhibition | ||||||
Function / homology | Function and homology information endopeptidase regulator activity / regulation of apoptosis involved in tissue homeostasis / inhibition of cysteine-type endopeptidase activity / positive regulation of protein linear polyubiquitination / regulation of BMP signaling pathway / copper ion homeostasis / nucleotide-binding oligomerization domain containing 1 signaling pathway / regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / protein serine/threonine kinase binding / nucleotide-binding oligomerization domain containing 2 signaling pathway ...endopeptidase regulator activity / regulation of apoptosis involved in tissue homeostasis / inhibition of cysteine-type endopeptidase activity / positive regulation of protein linear polyubiquitination / regulation of BMP signaling pathway / copper ion homeostasis / nucleotide-binding oligomerization domain containing 1 signaling pathway / regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / protein serine/threonine kinase binding / nucleotide-binding oligomerization domain containing 2 signaling pathway / SMAC, XIAP-regulated apoptotic response / Regulation of the apoptosome activity / Activation of caspases through apoptosome-mediated cleavage / SMAC (DIABLO) binds to IAPs / SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes / TNFR1-induced proapoptotic signaling / regulation of innate immune response / RIPK1-mediated regulated necrosis / cysteine-type endopeptidase inhibitor activity / protein K63-linked ubiquitination / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / positive regulation of type I interferon production / negative regulation of tumor necrosis factor-mediated signaling pathway / Regulation of PTEN localization / TNFR1-induced NF-kappa-B signaling pathway / positive regulation of protein ubiquitination / Deactivation of the beta-catenin transactivating complex / Regulation of TNFR1 signaling / positive regulation of JNK cascade / RING-type E3 ubiquitin transferase / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / Regulation of necroptotic cell death / Wnt signaling pathway / Regulation of PTEN stability and activity / ubiquitin-protein transferase activity / positive regulation of canonical Wnt signaling pathway / ubiquitin protein ligase activity / regulation of cell population proliferation / regulation of inflammatory response / neuron apoptotic process / regulation of apoptotic process / positive regulation of canonical NF-kappaB signal transduction / regulation of cell cycle / defense response to bacterium / DNA damage response / negative regulation of apoptotic process / nucleoplasm / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Sun, C. / Cai, M. / Meadows, R.P. / Fesik, S.W. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2000 Title: NMR structure and mutagenesis of the third Bir domain of the inhibitor of apoptosis protein XIAP. Authors: Sun, C. / Cai, M. / Meadows, R.P. / Xu, N. / Gunasekera, A.H. / Herrmann, J. / Wu, J.C. / Fesik, S.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f9x.cif.gz | 48.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f9x.ent.gz | 38.6 KB | Display | PDB format |
PDBx/mmJSON format | 1f9x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f9/1f9x ftp://data.pdbj.org/pub/pdb/validation_reports/f9/1f9x | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13707.228 Da / Num. of mol.: 1 / Fragment: RESIDUES 241-356 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET28B / Production host: Escherichia coli (E. coli) / References: UniProt: P98170 |
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#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.7 mM XIAP(residues 241-356)U-15N; 1.7 mM XIAP(residues 241-356)U-15N, 13C; Solvent system: 50 mM bis-Tris-d12 (pH=7.2), 300 mM KCl, 50 uM Zinc Acetate, 1 mM DTT; 95% H2O, 5% D2O |
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Sample conditions | Ionic strength: 300 mM KCl / pH: 7.2 / Pressure: 1 atm / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR ensemble | Conformers submitted total number: 1 |