[English] 日本語
![](img/lk-miru.gif)
- PDB-1f4s: STRUCTURE OF TRANSCRIPTIONAL FACTOR ALCR IN COMPLEX WITH A TARGET DNA -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1f4s | ||||||
---|---|---|---|---|---|---|---|
Title | STRUCTURE OF TRANSCRIPTIONAL FACTOR ALCR IN COMPLEX WITH A TARGET DNA | ||||||
![]() |
| ||||||
![]() | TRANSCRIPTION/DNA / PROTEIN-DNA COMPLEX / ZINC BINUCLEAR CLUSTER PROTEIN / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() acetaldehyde catabolic process / threonine catabolic process / ethanol catabolic process / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / DNA-binding transcription factor activity / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
![]() | Cahuzac, B. / Cerdan, R. / Felenbok, B. / Guittet, E. | ||||||
![]() | ![]() Title: The solution structure of an AlcR-DNA complex sheds light onto the unique tight and monomeric DNA binding of a Zn(2)Cys(6) protein. Authors: Cahuzac, B. / Cerdan, R. / Felenbok, B. / Guittet, E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 335.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 274.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 371.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 599 KB | Display | |
Data in XML | ![]() | 48.3 KB | Display | |
Data in CIF | ![]() | 64.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: DNA chain | Mass: 3061.003 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: ALCR CONSENSUS HALF-TARGET | ||
---|---|---|---|
#2: DNA chain | Mass: 3029.993 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: ALCR CONSENSUS HALF-TARGET | ||
#3: Protein | Mass: 7580.351 Da / Num. of mol.: 1 / Mutation: N-TERMINAL DNA-BINDING DOMAIN, RESIDUES 1-60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
#4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-
Sample preparation
Details | Contents: 1 mM AlcR U-15N, 1.1 mM double stranded DNA Solvent system: 20 mM phosphate buffer; 90%H2O, 10%D2O; pH 5.9 |
---|---|
Sample conditions | pH: 5.9 / Pressure: 1 atm / Temperature: 293 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-
Processing
NMR software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 Details: 1142 noe-derived constraints, 76 h-bond constraints, 33 phi angle restraints, 118 loose angle restraints on the DNA backbone | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 10 structures with the lowest energy. Conformers calculated total number: 20 / Conformers submitted total number: 10 |