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- PDB-1f3r: COMPLEX BETWEEN FV ANTIBODY FRAGMENT AND AN ANALOGUE OF THE MAIN ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f3r | ||||||
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Title | COMPLEX BETWEEN FV ANTIBODY FRAGMENT AND AN ANALOGUE OF THE MAIN IMMUNOGENIC REGION OF THE ACETYLCHOLINE RECEPTOR | ||||||
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![]() | IMMUNE SYSTEM / IG-FOLD / IMMUNO COMPLEX / ANTIBODY-ANTIGEN / BETA-TURN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : / : / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing docking molecular dynamics | ||||||
![]() | Kleinjung, J. / Petit, M.-C. / Orlewski, P. / Mamalaki, A. / Tzartos, S.-J. / Tsikaris, V. / Sakarellos-Daitsiotis, M. / Sakarellos, C. / Marraud, M. / Cung, M.-T. | ||||||
![]() | ![]() Title: The third-dimensional structure of the complex between an Fv antibody fragment and an analogue of the main immunogenic region of the acetylcholine receptor: a combined two-dimensional NMR, ...Title: The third-dimensional structure of the complex between an Fv antibody fragment and an analogue of the main immunogenic region of the acetylcholine receptor: a combined two-dimensional NMR, homology, and molecular modeling approach. Authors: Kleinjung, J. / Petit, M.C. / Orlewski, P. / Mamalaki, A. / Tzartos, S.J. / Tsikaris, V. / Sakarellos-Daitsiotis, M. / Sakarellos, C. / Marraud, M. / Cung, M.T. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.3 KB | Display | ![]() |
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PDB format | ![]() | 72 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 352 KB | Display | ![]() |
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Full document | ![]() | 357.8 KB | Display | |
Data in XML | ![]() | 7.4 KB | Display | |
Data in CIF | ![]() | 10.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1091.175 Da / Num. of mol.: 1 / Fragment: MAIN IMMUNOGENIC REGION (91-100) / Mutation: A70G, K76NLE / Source method: obtained synthetically Details: Sequence is derived from acetylcholine receptor of Torpedo electric organ References: GenBank: 223528 |
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#2: Antibody | Mass: 27775.750 Da / Num. of mol.: 1 Fragment: FRAGMENT OF MAB198 RAISED AGAINST HUMAN ACETYLCHOLINE RECEPTOR Source method: isolated from a natural source Details: MONOCLONAL ANTIBODY(MAB198) RAISED AGAINST HUMAN ACETYLCHOLINE RECEPTOR Source: (natural) ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
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Sample preparation
Details | Contents: 0.2 mM mAb198, 10 mM MIR peptide antigen, 0.1 M phosphate buffer Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 0.1 M / pH: 7.2 / Pressure: ambient / Temperature: 277 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 400 MHz |
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Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing docking molecular dynamics Software ordinal: 1 Details: The peptide structure is based on 73 NOE-derived distance restraints. The antibody structure is based on template structures Pot IgM and Fab D1.3 antilysozyme. The complex structure has been ...Details: The peptide structure is based on 73 NOE-derived distance restraints. The antibody structure is based on template structures Pot IgM and Fab D1.3 antilysozyme. The complex structure has been derived by computational docking. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 1 / Conformers submitted total number: 1 |