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Yorodumi- PDB-1f3k: THREE-DIMENSIONAL SOLUTION STRUCTURE OF OMEGA-CONOTOXIN TXVII, AN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f3k | ||||||
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Title | THREE-DIMENSIONAL SOLUTION STRUCTURE OF OMEGA-CONOTOXIN TXVII, AN L-TYPE CALCIUM CHANNEL BLOCKER | ||||||
Components | OMEGA-CONOTOXIN TXVII | ||||||
Keywords | TOXIN / OMEGA-CONOTOXIN / CYSTINE KNOT MOTIF / TRIPLE-STRANDED ANTIPARALLEL BETA-SHEET / L-TYPE CALCIUM CHANNEL BLOCKER | ||||||
Function / homology | Conotoxin, omega-type, conserved site / Omega-conotoxin family signature. / Conotoxin / Conotoxin / ion channel inhibitor activity / : / toxin activity / extracellular region / Omega-conotoxin TxVII Function and homology information | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
Authors | Kobayashi, K. / Sasaki, T. / Sato, K. / Kohno, T. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Three-dimensional solution structure of omega-conotoxin TxVII, an L-type calcium channel blocker. Authors: Kobayashi, K. / Sasaki, T. / Sato, K. / Kohno, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f3k.cif.gz | 126.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f3k.ent.gz | 102.4 KB | Display | PDB format |
PDBx/mmJSON format | 1f3k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f3/1f3k ftp://data.pdbj.org/pub/pdb/validation_reports/f3/1f3k | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2841.350 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This peptide was chemically synthesized. The sequence of this peptide naturally occurs in Conus textile. References: UniProt: P56714 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 5.6 / Pressure: ambient / Temperature: 288 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 452 restraints, 411 are NOE-derived distance constraints, 20 dihedral angle restraints, 21 distance restraints from hydrogen bonds and disulfide bonds. | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |