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Yorodumi- PDB-1f2v: CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE OF AEROBI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f2v | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF PRECORRIN-8X METHYLMUTASE OF AEROBIC VITAMIN B12 SYNTHESIS | ||||||
Components | PRECORRIN-8X METHYLMUTASE | ||||||
Keywords | ISOMERASE / alpha-beta wind / doubly wound sheet | ||||||
Function / homology | Function and homology information precorrin-8X methylmutase / precorrin-8X methylmutase activity / cobalamin biosynthetic process Similarity search - Function | ||||||
Biological species | Pseudomonas denitrificans (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | Shipman, L.W. / Li, D. / Roessner, C.A. / Scott, A.I. / Sacchettini, J.C. | ||||||
Citation | Journal: Structure / Year: 2001 Title: Crystal structure of precorrin-8x methyl mutase. Authors: Shipman, L.W. / Li, D. / Roessner, C.A. / Scott, A.I. / Sacchettini, J.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f2v.cif.gz | 55.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f2v.ent.gz | 39.4 KB | Display | PDB format |
PDBx/mmJSON format | 1f2v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1f2v_validation.pdf.gz | 426.8 KB | Display | wwPDB validaton report |
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Full document | 1f2v_full_validation.pdf.gz | 436.7 KB | Display | |
Data in XML | 1f2v_validation.xml.gz | 13.5 KB | Display | |
Data in CIF | 1f2v_validation.cif.gz | 19.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/1f2v ftp://data.pdbj.org/pub/pdb/validation_reports/f2/1f2v | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a dimer constructed from chain A and symmetry partner generated by the two-fold. |
-Components
#1: Protein | Mass: 23217.508 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas denitrificans (bacteria) / Plasmid: PLM1 / Production host: Escherichia coli (E. coli) / References: UniProt: P21638, precorrin-8X methylmutase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.18 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, magnesium chloride, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: May 18, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→30 Å / Num. all: 10108 / Num. obs: 8066 / % possible obs: 90.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.3 % / Biso Wilson estimate: 22.5 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 2.1→20 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.278 / % possible all: 94.1 |
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 30 Å / Num. measured all: 26519 |
Reflection shell | *PLUS % possible obs: 94.1 % / Mean I/σ(I) obs: 4.3 |
-Processing
Software |
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Refinement | Resolution: 2.1→20 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 20 Å / σ(F): 2 / Rfactor obs: 0.183 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |