+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1f2i | ||||||
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タイトル | COCRYSTAL STRUCTURE OF SELECTED ZINC FINGER DIMER BOUND TO DNA | ||||||
要素 |
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キーワード | TRANSCRIPTION/DNA / zinc finger (ジンクフィンガー) / dimer / protein-DNA complex (デオキシリボ核酸) / cooperativity / TRANSCRIPTION-DNA COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 glomerular mesangial cell proliferation / positive regulation of glomerular metanephric mesangial cell proliferation / cellular response to interleukin-8 / regulation of progesterone biosynthetic process / regulation of protein sumoylation / cellular response to mycophenolic acid / cellular response to heparin / circadian temperature homeostasis / positive regulation of post-translational protein modification / positive regulation of hormone biosynthetic process ...glomerular mesangial cell proliferation / positive regulation of glomerular metanephric mesangial cell proliferation / cellular response to interleukin-8 / regulation of progesterone biosynthetic process / regulation of protein sumoylation / cellular response to mycophenolic acid / cellular response to heparin / circadian temperature homeostasis / positive regulation of post-translational protein modification / positive regulation of hormone biosynthetic process / double-stranded methylated DNA binding / positive regulation of gene expression via chromosomal CpG island demethylation / hemi-methylated DNA-binding / interleukin-1-mediated signaling pathway / histone acetyltransferase binding / : / positive regulation of smooth muscle cell migration / skeletal muscle cell differentiation / locomotor rhythm / T cell differentiation / estrous cycle / RNA polymerase II core promoter sequence-specific DNA binding / BMP signaling pathway / long-term memory / response to glucose / regulation of neuron apoptotic process / positive regulation of chemokine production / positive regulation of interleukin-1 beta production / response to ischemia / promoter-specific chromatin binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / positive regulation of smooth muscle cell proliferation / regulation of long-term neuronal synaptic plasticity / circadian regulation of gene expression / response to insulin / negative regulation of canonical Wnt signaling pathway / cellular response to gamma radiation / positive regulation of miRNA transcription / positive regulation of neuron apoptotic process / double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / regulation of apoptotic process / sequence-specific DNA binding / learning or memory / transcription cis-regulatory region binding / response to hypoxia / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / クロマチン / regulation of DNA-templated transcription / positive regulation of gene expression / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / 核質 / 細胞核 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Mus musculus (ハツカネズミ) | ||||||
手法 | X線回折 / シンクロトロン / 解像度: 2.35 Å | ||||||
データ登録者 | Wang, B.S. / Grant, R.A. / Pabo, C.O. | ||||||
引用 | ジャーナル: Nat.Struct.Biol. / 年: 2001 タイトル: Selected peptide extension contacts hydrophobic patch on neighboring zinc finger and mediates dimerization on DNA. 著者: Wang, B.S. / Grant, R.A. / Pabo, C.O. #1: ジャーナル: Proc.Natl.Acad.Sci.USA / 年: 1999 タイトル: Dimerization of zinc fingers mediated by peptides evolved in vitro from random sequences 著者: Wang, B.S. / Pabo, C.O. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1f2i.cif.gz | 151.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1f2i.ent.gz | 112.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1f2i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/f2/1f2i ftp://data.pdbj.org/pub/pdb/validation_reports/f2/1f2i | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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2 |
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3 |
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単位格子 |
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詳細 | The biological assembly is a dimer constructed from chains A, B, G, and H / The biological assembly is a dimer constructed from chains C, D, I, and J / The biological assembly is a dimer constructed from chains E, F, K, and L |
-要素
#1: DNA鎖 | 分子量: 4281.780 Da / 分子数: 6 / 由来タイプ: 合成 #2: タンパク質 | 分子量: 8783.174 Da / 分子数: 6 / Fragment: ZIF12 CONTAINS ZINC FINGERS 1 AND 2 OF ZIF268 / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 遺伝子: GENE FOR ZIF12 / プラスミド: PET-21D / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P08046 #3: 化合物 | ChemComp-ZN / #4: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 2 |
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-試料調製
結晶 | マシュー密度: 3.59 Å3/Da / 溶媒含有率: 65.77 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.2 詳細: PEG 4000, NaCl, MgCl2, MES, pH 6.2, VAPOR DIFFUSION, HANGING DROP at 298K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 |
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結晶化 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 |
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放射光源 |
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検出器 |
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放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||||||||
放射波長 |
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反射 | 解像度: 2.35→20 Å / Num. all: 45864 / Num. obs: 45805 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / 冗長度: 4.2 % / Biso Wilson estimate: 24.7 Å2 / Rmerge(I) obs: 0.081 / Net I/σ(I): 15.5 | ||||||||||||||||||
反射 シェル | 解像度: 2.35→2.43 Å / 冗長度: 4 % / Rmerge(I) obs: 0.345 / Num. unique all: 4571 / % possible all: 99.8 | ||||||||||||||||||
反射 | *PLUS Num. measured all: 191628 | ||||||||||||||||||
反射 シェル | *PLUS % possible obs: 99.8 % |
-解析
ソフトウェア |
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精密化 | 解像度: 2.35→20 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 10000000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 2 / 立体化学のターゲット値: Engh & Huber
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原子変位パラメータ | Biso mean: 41.4 Å2 | ||||||||||||||||||||||||||||||||||||
Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.35→20 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.35→2.5 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 6
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Xplor file |
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ソフトウェア | *PLUS 名称: X-PLOR / バージョン: 3.851 / 分類: refinement | ||||||||||||||||||||||||||||||||||||
精密化 | *PLUS σ(F): 2 / % reflection Rfree: 10.1 % / Rfactor Rwork: 0.21 | ||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 41.4 Å2 | ||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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LS精密化 シェル | *PLUS Rfactor Rfree: 0.362 / % reflection Rfree: 11 % / Rfactor Rwork: 0.331 |