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Yorodumi- PDB-1eri: X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE-DNA RECOGNITION CO... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eri | |||||||||
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Title | X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE-DNA RECOGNITION COMPLEX: THE RECOGNITION NETWORK AND THE INTEGRATION OF RECOGNITION AND CLEAVAGE | |||||||||
Components |
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Keywords | HYDROLASE/DNA / PROTEIN-DNA COMPLEX / DOUBLE HELIX / HYDROLASE-DNA COMPLEX | |||||||||
Function / homology | Function and homology information type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / magnesium ion binding / DNA binding Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | |||||||||
Authors | Kim, Y. / Grable, J.C. / Love, R. / Greene, P.J. / Rosenberg, J.M. | |||||||||
Citation | Journal: Science / Year: 1990 Title: Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing. Authors: Kim, Y.C. / Grable, J.C. / Love, R. / Greene, P.J. / Rosenberg, J.M. #1: Journal: To be Published Title: Preliminary Characterization of EcoRI-DNA Co-Crystals: Incomplete Factorial Design of Oligonucleotide Sequences Authors: Wilkosz, P.A. / Chandrasekhar, K. / Rosenberg, J.M. #2: Journal: To be Published Title: Molecular Dynamics Simulations Suggest that the EcoRI Kink is an Example of Molecular Strain Authors: Kumar, S. / Duan, Y. / Kollman, P.A. / Rosenberg, J.M. #3: Journal: Structural Biology: The State of the Art; Proceedings of the Eighth Conversation, State University of New York, Albany Year: 1994 Title: Studies on the Canonical DNA-EcoRI Endonuclease Complex and the EcoRI Kink Authors: Kim, Y. / Choi, J. / Grable, J.C. / Greene, P. / Hager, P. / Rosenberg, J.M. #4: Journal: Curr.Opin.Struct.Biol. / Year: 1991 Title: Structure and Function of Restriction Endonucleases Authors: Rosenberg, J.M. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eri.cif.gz | 74.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eri.ent.gz | 52.9 KB | Display | PDB format |
PDBx/mmJSON format | 1eri.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/er/1eri ftp://data.pdbj.org/pub/pdb/validation_reports/er/1eri | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3967.585 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: Protein | Mass: 30970.184 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P00642 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 58 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Details: 277.00K | |||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal | *PLUS | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusionDetails: Grable J., (1984) J. Biomol. Struct. Dyn., 1, 1149. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 2.7 Å / Num. obs: 8775 / % possible obs: 60 % / Observed criterion σ(I): 3 |
Reflection | *PLUS Highest resolution: 2.7 Å / % possible obs: 60 % / Observed criterion σ(I): 3 |
-Processing
Software |
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Refinement | Resolution: 2.5→8 Å / σ(F): 3
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Displacement parameters | Biso mean: 26.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→8 Å
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Refine LS restraints |
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