+Open data
-Basic information
Entry | Database: PDB / ID: 1enh | ||||||
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Title | STRUCTURAL STUDIES OF THE ENGRAILED HOMEODOMAIN | ||||||
Components | ENGRAILED HOMEODOMAIN | ||||||
Keywords | DNA BINDING PROTEIN / DNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information posterior compartment specification / analia development / anterior head segmentation / posterior head segmentation / anterior/posterior lineage restriction, imaginal disc / trunk segmentation / genital disc development / genital disc anterior/posterior pattern formation / spiracle morphogenesis, open tracheal system / wing disc anterior/posterior pattern formation ...posterior compartment specification / analia development / anterior head segmentation / posterior head segmentation / anterior/posterior lineage restriction, imaginal disc / trunk segmentation / genital disc development / genital disc anterior/posterior pattern formation / spiracle morphogenesis, open tracheal system / wing disc anterior/posterior pattern formation / wing disc morphogenesis / neuroblast fate determination / imaginal disc-derived wing vein specification / segment polarity determination / ventral midline development / compartment pattern specification / gonad development / RSC-type complex / RNA polymerase II transcription regulatory region sequence-specific DNA binding / axon guidance / neuron differentiation / DNA-binding transcription repressor activity, RNA polymerase II-specific / regulation of gene expression / negative regulation of neuron apoptotic process / sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of gene expression / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | Clarke, N.D. / Kissinger, C.R. / Desjarlais, J. / Gilliland, G.L. / Pabo, C.O. | ||||||
Citation | Journal: Protein Sci. / Year: 1994 Title: Structural studies of the engrailed homeodomain. Authors: Clarke, N.D. / Kissinger, C.R. / Desjarlais, J. / Gilliland, G.L. / Pabo, C.O. #1: Journal: Cell(Cambridge,Mass.) / Year: 1990 Title: Crystal Structure of an Engrailed Homeodomain-DNA Complex at 2.8 Resolution: A Framework for Understanding Homeodomain-DNA Interactions Authors: Kissinger, C.R. / Liu, B. / Martin-Blanco, E. / Kornberg, T.B. / Pabo, C.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1enh.cif.gz | 21.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1enh.ent.gz | 13.5 KB | Display | PDB format |
PDBx/mmJSON format | 1enh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1enh_validation.pdf.gz | 351.9 KB | Display | wwPDB validaton report |
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Full document | 1enh_full_validation.pdf.gz | 353.4 KB | Display | |
Data in XML | 1enh_validation.xml.gz | 2.4 KB | Display | |
Data in CIF | 1enh_validation.cif.gz | 3.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/en/1enh ftp://data.pdbj.org/pub/pdb/validation_reports/en/1enh | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6619.536 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / References: UniProt: P02836 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.13 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.7 / Method: vapor diffusion, hanging dropDetails: referred to 'Liu B.', (1990) 'Biochem. Biophys. Res. Commun.', 171, 257-259 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.89 Å / Num. obs: 6104 / % possible obs: 86.8 % / Num. measured all: 21567 / Rmerge(I) obs: 0.0375 |
-Processing
Software |
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Refinement | Resolution: 2.1→8 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.1→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.197 / Highest resolution: 2.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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