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Yorodumi- PDB-1emw: SOLUTION STRUCTURE OF THE RIBOSOMAL PROTEIN S16 FROM THERMUS THER... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1emw | ||||||
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Title | SOLUTION STRUCTURE OF THE RIBOSOMAL PROTEIN S16 FROM THERMUS THERMOPHILUS | ||||||
Components | S16 RIBOSOMAL PROTEIN | ||||||
Keywords | RIBOSOME / MIXED ALPHA/BETA PROTEIN | ||||||
Function / homology | Function and homology information rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / cytoplasm Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Allard, P. / Rak, A.V. / Wimberly, B.T. / Clemons Jr., W.M. / Kalinin, A. / Helgstrand, M. / Garber, M.B. / Ramakrishnan, V. / Hard, T. | ||||||
Citation | Journal: Structure Fold.Des. / Year: 2000 Title: Another piece of the ribosome: solution structure of S16 and its location in the 30S subunit. Authors: Allard, P. / Rak, A.V. / Wimberly, B.T. / Clemons Jr., W.M. / Kalinin, A. / Helgstrand, M. / Garber, M.B. / Ramakrishnan, V. / Hard, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1emw.cif.gz | 1.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1emw.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 1emw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/em/1emw ftp://data.pdbj.org/pub/pdb/validation_reports/em/1emw | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10409.983 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Plasmid: PET11C / Production host: Escherichia coli (E. coli) / References: UniProt: P80379 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 0.2 M LiCl / pH: 6.0 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: 1283 NOE-derived distance constraints, 47 dihedral angle restraints,31 distance restraints from hydrogen bonds. | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 47 |