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Yorodumi- PDB-1eik: Solution Structure of RNA Polymerase Subunit RPB5 from Methanobac... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1eik | ||||||
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Title | Solution Structure of RNA Polymerase Subunit RPB5 from Methanobacterium Thermoautotrophicum | ||||||
Components | RNA POLYMERASE SUBUNIT RPB5 | ||||||
Keywords | TRANSFERASE / rpb5 / rpbH / rna polymerase subunit / OCSP / NESG / PROTEIN STRUCTURE INITIATIVE / Structural Genomics / PSI / Northeast Structural Genomics Consortium | ||||||
Function / homology | Function and homology information DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / DNA-templated transcription / DNA binding Similarity search - Function | ||||||
Biological species | Methanothermobacter thermautotrophicus (archaea) | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
Authors | Yee, A. / Booth, V. / Dharamsi, A. / Engel, A. / Edwards, A.M. / Arrowsmith, C.H. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2000 Title: Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum. Authors: Yee, A. / Booth, V. / Dharamsi, A. / Engel, A. / Edwards, A.M. / Arrowsmith, C.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1eik.cif.gz | 252.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1eik.ent.gz | 217.9 KB | Display | PDB format |
PDBx/mmJSON format | 1eik.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ei/1eik ftp://data.pdbj.org/pub/pdb/validation_reports/ei/1eik | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8826.354 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanothermobacter thermautotrophicus (archaea) Plasmid: PET15B / Production host: Escherichia coli (E. coli) / References: UniProt: O27122, DNA-directed RNA polymerase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy |
-Sample preparation
Details | Contents: u-15n, 13c; 150 mM NaCl, 25 mM phosphate, 10% D2O, pH 6.5 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 150 mM NaCl / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 |