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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1ebh | ||||||
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タイトル | OCTAHEDRAL COORDINATION AT THE HIGH AFFINITY METAL SITE IN ENOLASE; CRYSTALLOGRAPHIC ANALYSIS OF THE MG++-ENZYME FROM YEAST AT 1.9 ANGSTROMS RESOLUTION | ||||||
![]() | ENOLASE | ||||||
![]() | CARBON-OXYGEN LYASE | ||||||
機能・相同性 | ![]() Gluconeogenesis / regulation of vacuole fusion, non-autophagic / Glycolysis / melatonin binding / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / fungal-type vacuole / glycolytic process / magnesium ion binding ...Gluconeogenesis / regulation of vacuole fusion, non-autophagic / Glycolysis / melatonin binding / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / fungal-type vacuole / glycolytic process / magnesium ion binding / mitochondrion / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Wedekind, J.E. / Reed, G.H. / Rayment, I. | ||||||
![]() | ![]() タイトル: Octahedral coordination at the high-affinity metal site in enolase: crystallographic analysis of the MgII--enzyme complex from yeast at 1.9 A resolution. 著者: Wedekind, J.E. / Reed, G.H. / Rayment, I. #1: ![]() タイトル: Chelation of Ser 39 to Mg++ Latches a Gate at the Active Site of Enolase: Structure of the Bis Mg++ Complex of Yeast Enolase and the Intermediate, Phosphonoacetohydroxamate, at 2.1 Angstroms Resolution 著者: Wedekind, J.E. / Reed, G.H. / Rayment, I. | ||||||
履歴 |
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Remark 700 | SHEET THE SHEETS PRESENTED AS *BAA* AND *BAB* ON SHEET RECORDS BELOW ARE ACTUALLY EIGHT-STRANDED ...SHEET THE SHEETS PRESENTED AS *BAA* AND *BAB* ON SHEET RECORDS BELOW ARE ACTUALLY EIGHT-STRANDED BETA-BARRELS. THESE ARE REPRESENTED BY NINE-STRANDED SHEETS IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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PDBx/mmCIF形式 | ![]() | 185.7 KB | 表示 | ![]() |
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PDB形式 | ![]() | 146 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: CIS PROLINE - PRO A 143 / 2: CIS PROLINE - PRO B 143 | ||||||||
非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (0.49366, -1.0E-5, -0.86965), ベクター: 詳細 | THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW WILL YIELD APPROXIMATE COORDINATES FOR CHAIN A WHEN APPLIED TO CHAIN B. THIS TRANSFORMATION IS A TWO-FOLD OPERATION AND TRANSLATION THAT DESCRIBE THE APPROXIMATE NON-CRYSTALLOGRAPHIC DYAD. THE CRYSTALLOGRAPHICALLY INDEPENDENT UNIT IS ONE DIMER OF CHEMICALLY IDENTICAL SUBUNITS. | |
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要素
#1: タンパク質 | 分子量: 46732.797 Da / 分子数: 2 / 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() 参照: UniProt: P00924, phosphopyruvate hydratase #2: 化合物 | #3: 化合物 | #4: 水 | ChemComp-HOH / | 構成要素の詳細 | THIS STRUCTURE COMPLEMENTS THE STUDY OF ENOLASE-BIS(MG)II-PHAH AND SERVES AS A CONTROL EXPERIMENT ...THIS STRUCTURE COMPLEMENT | 非ポリマーの詳細 | METALS ARE HEXACOORDINATE WITH OCTAHEDRAL GEOMETRY. A CHLORIDE ION IS BOUND AT THE PHOSPHATE ...METALS ARE HEXACOORDI | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.45 Å3/Da / 溶媒含有率: 49.78 % | ||||||||||||||||||||||||||||||
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結晶化 | 詳細: CRYSTALS WERE GROWN FROM POLYETHYLENE GLYCOL, KCL, AT PH 8.1. CRYSTALLIZED IN THE PRESENCE OF 0.5 MILLIMOLAR MG2+ | ||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 8 / 手法: batch method | ||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
反射 | *PLUS 最高解像度: 1.9 Å / 最低解像度: 100 Å / Num. obs: 53410 / % possible obs: 75 % / Num. measured all: 77897 / Rmerge(I) obs: 0.041 |
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反射 シェル | *PLUS 最高解像度: 1.9 Å / 最低解像度: 2.02 Å / Num. unique obs: 6194 / Num. measured obs: 6392 / Rmerge(I) obs: 0.29 |
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解析
ソフトウェア |
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精密化 | 解像度: 1.9→50 Å / σ(F): 0 詳細: OCCUPANCIES FOR RESIDUES EXHIBITING DUAL CONFORMATIONS HAVE TOTAL OCCUPANCIES OF 1.0 WITH THE FOLLOWING EXCEPTION. ALA A 38 AND ALA B 38 WERE MODELED IN THE OPEN CONFORMATION ONL AFTER ATOM N ...詳細: OCCUPANCIES FOR RESIDUES EXHIBITING DUAL CONFORMATIONS HAVE TOTAL OCCUPANCIES OF 1.0 WITH THE FOLLOWING EXCEPTION. ALA A 38 AND ALA B 38 WERE MODELED IN THE OPEN CONFORMATION ONL AFTER ATOM N ALA 38 IN EACH CHAIN. THE TOTAL OCCUPANCIES FOR ATOMS OF THIS RESIDUE TOTAL 0.7.
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精密化ステップ | サイクル: LAST / 解像度: 1.9→50 Å
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拘束条件 |
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精密化 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 34 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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