+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1eao | ||||||
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タイトル | THE RUNX1 Runt domain at 1.4A resolution: a structural switch and specifically bound chloride ions modulate DNA binding | ||||||
要素 | RUNT-RELATED TRANSCRIPTION FACTOR 1 | ||||||
キーワード | TRANSCRIPTION/DNA / ACUTE MYELOID LEUKEMIA / AML / RUNX1 / RUNT DOMAIN / CHLORIDE BINDING / TRANSCRIPTION FACTOR / IG FOLD / TRANSCRIPTION-DNA complex | ||||||
機能・相同性 | 機能・相同性情報 regulation of hair follicle cell proliferation / Organic cation transport / positive regulation of progesterone secretion / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / positive regulation of granulocyte differentiation ...regulation of hair follicle cell proliferation / Organic cation transport / positive regulation of progesterone secretion / RUNX1 regulates estrogen receptor mediated transcription / RUNX1 regulates transcription of genes involved in BCR signaling / RUNX1 regulates transcription of genes involved in interleukin signaling / Regulation of RUNX1 Expression and Activity / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / positive regulation of granulocyte differentiation / core-binding factor complex / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / positive regulation of CD8-positive, alpha-beta T cell differentiation / positive regulation of cell maturation / negative regulation of CD4-positive, alpha-beta T cell differentiation / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RUNX1 regulates transcription of genes involved in differentiation of HSCs / neuron fate commitment / Estrogen-dependent gene expression / myeloid progenitor cell differentiation / definitive hemopoiesis / regulation of T cell anergy / embryonic hemopoiesis / hair follicle morphogenesis / regulation of cell differentiation / behavioral response to pain / hemopoiesis / basement membrane / regulation of signal transduction / neuron development / chondrocyte differentiation / response to retinoic acid / cellular response to transforming growth factor beta stimulus / positive regulation of interleukin-2 production / ossification / liver development / skeletal system development / central nervous system development / promoter-specific chromatin binding / neuron differentiation / positive regulation of angiogenesis / positive regulation of type II interferon production / sequence-specific double-stranded DNA binding / DNA-binding transcription factor binding / in utero embryonic development / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / ATP binding / nucleus 類似検索 - 分子機能 | ||||||
生物種 | MUS MUSCULUS (ハツカネズミ) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.4 Å | ||||||
データ登録者 | Backstrom, S. / Wolf-Watz, M. / Grundstrom, C. / Hard, T.H. / Grundstrom, T. / Sauer, U.H. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 2002 タイトル: The Runx1 Runt Domain at 1.25A Resolution: A Structural Switch and Specifically Bound Chloride Ions Modulate DNA Binding 著者: Backstrom, S. / Wolf-Watz, M. / Grundstrom, C. / Hard, T. / Grundstrom, T. / Sauer, U. | ||||||
履歴 |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1eao.cif.gz | 122.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1eao.ent.gz | 96 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1eao.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ea/1eao ftp://data.pdbj.org/pub/pdb/validation_reports/ea/1eao | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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2 |
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (-0.756729, -0.297093, -0.58232), ベクター: |
-要素
#1: タンパク質 | 分子量: 15523.550 Da / 分子数: 2 / 断片: RUNT DOMAIN RESIDUES 46-185 / 変異: YES / 由来タイプ: 組換発現 / 詳細: ENGINEERED MUTATION CYS 72 SER, CYS 81 SER / 由来: (組換発現) MUS MUSCULUS (ハツカネズミ) / 解説: REFOLDED FROM INCLUSION BODIES / プラスミド: PET11C / 発現宿主: ESCHERICHIA COLI (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: Q03347 #2: 化合物 | ChemComp-BR / #3: 水 | ChemComp-HOH / | 構成要素の詳細 | CHAIN A ENGINEERED MUTATION CYS 72 SER, CYS 81 SER CBF BINDS TO THE CORE SITE, OF A NUMBER OF ...CHAIN A ENGINEERED | |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.2 Å3/Da / 溶媒含有率: 34 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 6.4 詳細: 25 % PEG 3350, 16% GLYCEROL, 130 MM NA CACODYLATE, PH 6.4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 8 / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: ESRF / ビームライン: ID14-4 / 波長: 0.9195 |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2000年4月15日 / 詳細: MIRROR |
放射 | モノクロメーター: SI(111), SI(311) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9195 Å / 相対比: 1 |
反射 | 解像度: 1.4→24 Å / Num. obs: 50436 / % possible obs: 97.5 % / Observed criterion σ(I): 0 / 冗長度: 4.45 % / Rmerge(I) obs: 0.079 / Net I/σ(I): 10.9 |
反射 シェル | 解像度: 1.4→1.45 Å / 冗長度: 4 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 4 / % possible all: 96.4 |
反射 | *PLUS 最低解像度: 24 Å / Num. measured all: 224261 |
反射 シェル | *PLUS % possible obs: 97 % |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: EBI-8233 解像度: 1.4→24.77 Å / Cor.coef. Fo:Fc: 0.967 / Cor.coef. Fo:Fc free: 0.955 / SU B: 0.698 / SU ML: 0.029 / 交差検証法: THROUGHOUT / ESU R: 0.059 / ESU R Free: 0.054 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 15.01 Å2
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精密化ステップ | サイクル: LAST / 解像度: 1.4→24.77 Å
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拘束条件 |
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