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- PDB-1e07: Model of human carcinoembryonic antigen by homology modelling and... -
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Basic information
Entry | Database: PDB / ID: 10000000 | ||||||
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Title | Model of human carcinoembryonic antigen by homology modelling and curve-fitting to experimental solution scattering data | ||||||
![]() | CARCINOEMBRYONIC ANTIGEN | ||||||
![]() | GLYCOPROTEIN / CEA / TUMOUR MARKER / IMMUNOGLOBULIN-FOLD | ||||||
Function / homology | ![]() GPI anchor binding / homotypic cell-cell adhesion / Post-translational modification: synthesis of GPI-anchored proteins / negative regulation of myotube differentiation / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / homophilic cell adhesion via plasma membrane adhesion molecules / negative regulation of anoikis / side of membrane / Cell surface interactions at the vascular wall / basolateral plasma membrane ...GPI anchor binding / homotypic cell-cell adhesion / Post-translational modification: synthesis of GPI-anchored proteins / negative regulation of myotube differentiation / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / homophilic cell adhesion via plasma membrane adhesion molecules / negative regulation of anoikis / side of membrane / Cell surface interactions at the vascular wall / basolateral plasma membrane / apical plasma membrane / negative regulation of apoptotic process / apoptotic process / cell surface / protein homodimerization activity / extracellular exosome / extracellular region / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
Model type details | CA ATOMS ONLY, CHAIN A | ||||||
![]() | Boehm, M.K. / Perkins, S.J. | ||||||
![]() | ![]() Title: Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23. Authors: Boehm, M.K. / Perkins, S.J. #1: ![]() Title: Crystal Structure of the Anti-(Carcinoembryonic Antigen) Single-Chain Fv Antibody Mfe-23 and a Model for Antigen Binding Based on Intermolecular Contacts. Authors: Boehm, M.K. / Corper, A.L. / Wan, T. / Sohi, M.K. / Sutton, B.J. / Thornton, J.D. / Keep, P.A. / Chester, K.A. / Begent, R.H. / Perkins, S.J. #2: Journal: J.Mol.Biol. / Year: 1996 Title: Extended Glycoprotein Structure of the Seven Domains in Human Carcinoembryonic Antigen by X-Ray and Neutron Solution Scattering and an Automated Curve Fitting Procedure: Implications for Cellular Adhesion. Authors: Boehm, M.K. / Mayans, M.O. / Thornton, J.D. / Begent, R.H. / Keep, P.A. / Perkins, S.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 31.3 KB | Display | ![]() |
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PDB format | ![]() | 15.7 KB | Display | ![]() |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 70620.727 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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Sequence details | THE CARCINOEMBRYONIC ANTIGEN PRECURSOR CONTAINS A 34-RESIDUE N-TERMINAL SIGNAL PEPTIDE AND A 26- ...THE CARCINOEMB |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | *PLUS Method: other / Details: solution scattering |
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-Data collection
Soln scatter |
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Processing
Software | Name: DISCOVERY / Classification: refinement | ||||||||||||
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Refinement | Details: ENERGY MINIMIZATION. DETAILS ARE GIVEN IN THE PRIMARY REFERENCE. | ||||||||||||
Refinement step | Cycle: LAST
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Soln scatter model | Method: CONSTRAINED SCATTERING FITTING OF HOMOLOGY MODELS Details: HUMAN CARCINOEMBRYONIC ANTIGEN (CEA) CONTAINS SEVEN EXTRACELLULAR IMMUNOGLOBULIN (IG) FOLD DOMAINS. THE N- TERMINAL DOMAIN IS PREDICTED TO HAVE A V-TYPE IG FOLD, AND WAS MODELLED USING THE V- ...Details: HUMAN CARCINOEMBRYONIC ANTIGEN (CEA) CONTAINS SEVEN EXTRACELLULAR IMMUNOGLOBULIN (IG) FOLD DOMAINS. THE N- TERMINAL DOMAIN IS PREDICTED TO HAVE A V-TYPE IG FOLD, AND WAS MODELLED USING THE V-TYPE DOMAIN STRUCTURES FROM HUMAN CD2 (CODE: 1HNF), HUMAN CD4 (CODE: 3CD4) AND HUMAN CD8 (CODE: 1CD8) . THE SECOND, FOURTH AND SIXTH DOMAINS (COMMONLY REFERRED TO AS IA, IIA AND IIIA) ARE PREDICTED TO HAVE I-TYPE IG FOLDS, AND WERE MODELLED USING THE I-TYPE DOMAIN STRUCTURE FROM HUMAN VCAM-1 (CODE: 1VCA). THE THIRD, FIFTH AND SEVENTH DOMAINS (COMMONLY REFERRED TO AS IB, IIB AND IIIB) ARE PREDICTED TO HAVE C2-TYPE IG FOLDS, AND WERE MODELLED USING THE C2-TYPE DOMAIN STRUCTURE FROM HUMAN CD2 (CODE: 1HNF). FURTHER DETAILS OF THE HOMOLOGY MODELLING ARE GIVEN IN THE PRIMARY REFERENCE. IN THE COMPLETE CEA MODEL, ALL INTER-DOMAIN ORIENTATIONS BETWEEN NEIGHBOURING DOMAINS ARE BASED ON THAT BETWEEN THE TWO DOMAINS IN HUMAN CD2. SUCH AN ARRANGEMENT OF THE DOMAINS WAS SHOWN TO BE COMPATIBLE WITH THE SOLUTION STRUCTURE OF CEA BY X- RAY AND NEUTRON SCATTERING (SEE REFERENCE TWO). Entry fitting list: PDB CODE 3CD4, 1CD8 / Num. of conformers submitted: 1 / Software author list: BIOSYM/MSI / Software list: INSIGHT II, HOMOLOGY, SCTPL4, GNOM |