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Yorodumi- PDB-1dvv: SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dvv | ||||||
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Title | SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA | ||||||
Components | CYTOCHROME C551 | ||||||
Keywords | ELECTRON TRANSPORT / cytochrome c / stability | ||||||
Function / homology | Function and homology information electron transfer activity / periplasmic space / iron ion binding / heme binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hasegawa, J. / Uchiyama, S. / Tanimoto, Y. / Mizutani, M. / Kobayashi, Y. / Sambongi, Y. / Igarashi, Y. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2000 Title: Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart. Authors: Hasegawa, J. / Uchiyama, S. / Tanimoto, Y. / Mizutani, M. / Kobayashi, Y. / Sambongi, Y. / Igarashi, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dvv.cif.gz | 501.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dvv.ent.gz | 435.9 KB | Display | PDB format |
PDBx/mmJSON format | 1dvv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dv/1dvv ftp://data.pdbj.org/pub/pdb/validation_reports/dv/1dvv | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8724.947 Da / Num. of mol.: 1 / Mutation: F7A, V13M, F34Y, E43Y, V78I Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P00099 |
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#2: Chemical | ChemComp-HEM / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details |
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Sample conditions | pH: 5.0 / Pressure: 1 atm / Temperature: 298 K | |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software | Name: X-PLOR / Version: 3.1 / Developer: Brunger / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |