- PDB-1duj: SOLUTION STRUCTURE OF THE SPINDLE ASSEMBLY CHECKPOINT PROTEIN HUM... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 1duj
Title
SOLUTION STRUCTURE OF THE SPINDLE ASSEMBLY CHECKPOINT PROTEIN HUMAN MAD2
Components
SPINDLE ASSEMBLY CHECKPOINT PROTEIN
Keywords
CELL CYCLE / Mad2 / spindle assembly checkpoint
Function / homology
Function and homology information
mitotic spindle assembly checkpoint MAD1-MAD2 complex / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / mitotic checkpoint complex / positive regulation of mitotic cell cycle spindle assembly checkpoint / establishment of centrosome localization / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / nuclear pore nuclear basket / negative regulation of ubiquitin protein ligase activity / mitotic spindle assembly checkpoint signaling ...mitotic spindle assembly checkpoint MAD1-MAD2 complex / Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components / mitotic checkpoint complex / positive regulation of mitotic cell cycle spindle assembly checkpoint / establishment of centrosome localization / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / nuclear pore nuclear basket / negative regulation of ubiquitin protein ligase activity / mitotic spindle assembly checkpoint signaling / establishment of mitotic spindle orientation / mitotic sister chromatid segregation / negative regulation of mitotic cell cycle / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / APC-Cdc20 mediated degradation of Nek2A / RHO GTPases Activate Formins / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / negative regulation of protein catabolic process / mitotic spindle / kinetochore / spindle pole / Separation of Sister Chromatids / cell division / negative regulation of apoptotic process / perinuclear region of cytoplasm / protein homodimerization activity / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function
Cell Cycle, Spindle Assembly Checkpoint Protein; Chain A / HORMA domain / Mad2-like / HORMA domain / HORMA domain / HORMA domain profile. / HORMA domain superfamily / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 21430.369 Da / Num. of mol.: 1 Fragment: FULL PROTEIN WITHOUT BOTH N- AND C-TERMINAL 10 RESIDUES Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PQE-30 / Production host: Escherichia coli (E. coli) / References: UniProt: Q13257
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
3
1
3
3D 15N-separated NOESY
5
2
5
3D 13C-separated NOESY
2
3
2
3D (H)CCH-TOCSY
3
4
3
3D 15N-separated TOCSY
2
5
2
3DH(CC)(CO)NH
2
6
2
3D (H)C(C)(CO)NH
NMR details
Text: The structure was determined using triple-resonance NMR spectroscopy.
Method: simulated annealing / Software ordinal: 1 Details: The structures are based on a total of 3111 restraints, 2807 are NOE-derived distance constraints, 214 dihedral angle restraints,90 distance restraints from hydrogen bonds.
NMR ensemble
Conformers submitted total number: 1
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