ACETOACETYL-COA IS THE MOST EFFECTIVE INHIBITOR OF THE ENOYL-COA HYDRATASE REACTION THAT IS DESCRIBED.
配列の詳細
A 29 AMINO ACID N-TERMINAL LEADING SEQUENCE OF THE PROTEIN IS CLEAVED OFF UPON IMPORT INTO THE ...A 29 AMINO ACID N-TERMINAL LEADING SEQUENCE OF THE PROTEIN IS CLEAVED OFF UPON IMPORT INTO THE MITOCHONDRION. THE RESIDUE NUMBERING SCHEME IS ADAPTED FROM THE GENE SEQUENCE AND STARTS THEREFORE WITH GLY 30.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.59 Å3/Da / 溶媒含有率: 53 %
結晶化
pH: 6.5 詳細: 100 MM MES/NAOH PH 6.5, 2.5 M AMMONIUM SULFATE, 10% OCTANOL, 1 MM DTT, 1 MM EDTA, 1 MM NA-AZIDE, 1 MM ACETOACETYL-COA.
解像度: 2.5→28.6 Å / Num. obs: 58197 / % possible obs: 95.7 % / Observed criterion σ(I): 0 / 冗長度: 4.2 % / Biso Wilson estimate: 22.77 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 14.3
反射 シェル
解像度: 2.5→2.59 Å / 冗長度: 3.7 % / Rmerge(I) obs: 0.18 / Mean I/σ(I) obs: 5.6 / % possible all: 97.8
反射
*PLUS
Num. measured all: 243398
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解析
ソフトウェア
名称
バージョン
分類
MLPHARE
位相決定
X-PLOR
3.1
精密化
DENZO
データ削減
精密化
構造決定の手法: 多重同系置換 / 解像度: 2.5→8 Å / 交差検証法: R-FREE / σ(F): 0 詳細: ONLY 5 OF THE 6 SUBUNITS IN THE ASYMMETRIC UNIT HAVE A LIGAND BOUND. IN THE BINDING SITE OF SUBUNIT D A CLASH OF THE 3'-PHOSPHATE OF ACETO-ACETYL-COA WITH THE LOOP LYS 41 TO SER 45 OF A ...詳細: ONLY 5 OF THE 6 SUBUNITS IN THE ASYMMETRIC UNIT HAVE A LIGAND BOUND. IN THE BINDING SITE OF SUBUNIT D A CLASH OF THE 3'-PHOSPHATE OF ACETO-ACETYL-COA WITH THE LOOP LYS 41 TO SER 45 OF A CRYSTALLOGRAPHIC SYMMETRY RELATED D SUBUNITS PREVENTS THE LIGAND FROM BINDING.