+Open data
-Basic information
Entry | Database: PDB / ID: 1dqz | ||||||
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Title | CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS | ||||||
Components | PROTEIN (ANTIGEN 85-C) | ||||||
Keywords | IMMUNE SYSTEM / ANTIGEN / 85C / MYCOBACTERIUM TUBERCULOSIS / FIBRONECTIN / Structural Genomics / PSI / Protein Structure Initiative / TB Structural Genomics Consortium / TBSGC | ||||||
Function / homology | Function and homology information trehalose O-mycolyltransferase / trehalose O-mycolyltransferase activity / diacylglycerol O-acyltransferase / diacylglycerol O-acyltransferase activity / glycolipid biosynthetic process / mycolate cell wall layer assembly / zymogen binding / lipid transport / acyltransferase activity, transferring groups other than amino-acyl groups / peptidoglycan-based cell wall ...trehalose O-mycolyltransferase / trehalose O-mycolyltransferase activity / diacylglycerol O-acyltransferase / diacylglycerol O-acyltransferase activity / glycolipid biosynthetic process / mycolate cell wall layer assembly / zymogen binding / lipid transport / acyltransferase activity, transferring groups other than amino-acyl groups / peptidoglycan-based cell wall / response to antibiotic / extracellular region Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.5 Å | ||||||
Authors | Ronning, D.R. / Klabunde, T. / Sacchettini, J.C. / TB Structural Genomics Consortium (TBSGC) | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines. Authors: Ronning, D.R. / Klabunde, T. / Besra, G.S. / Vissa, V.D. / Belisle, J.T. / Sacchettini, J.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dqz.cif.gz | 130 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dqz.ent.gz | 101 KB | Display | PDB format |
PDBx/mmJSON format | 1dqz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dq/1dqz ftp://data.pdbj.org/pub/pdb/validation_reports/dq/1dqz | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 30849.123 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mycobacterium tuberculosis (bacteria) / References: UniProt: P0A4V4, UniProt: P9WQN9*PLUS #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 57.04 % | ||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 53 % | ||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.6 / Method: vapor diffusion, hanging drop / Details: refer to Mueller, U., (1999) RNA, 5, 670. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.5 Å / Lowest resolution: 20 Å / Num. obs: 110059 / % possible obs: 96.9 % / Num. measured all: 425433 / Rmerge(I) obs: 0.045 |
Reflection shell | *PLUS % possible obs: 99.2 % / Rmerge(I) obs: 0.276 |
-Processing
Software |
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Refinement | Resolution: 1.5→30 Å / σ(I): 1
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.5→30 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 19.83 Å2 | ||||||||||||||||||||
Refine LS restraints | *PLUS
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