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- PDB-1dmu: Crystal structure of the restriction endonuclease BglI (e.c.3.1.2... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1dmu | ||||||
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Title | Crystal structure of the restriction endonuclease BglI (e.c.3.1.21.4) bound to its dna recognition sequence | ||||||
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![]() | HYDROLASE/DNA / PROTEIN-DNA COMPLEX / ACTIVE SITE CALCIUM IONS / ALPHA/BETA STRUCTURE / A:A MISMATCH / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | ![]() type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Newman, M. / Lunnen, K. / Wilson, G. / Greci, J. / Schildkraut, I. / Phillips, S.E.V. | ||||||
![]() | ![]() Title: Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence. Authors: Newman, M. / Lunnen, K. / Wilson, G. / Greci, J. / Schildkraut, I. / Phillips, S.E. #1: ![]() Title: Method for producing the BglI restriction endonuclease and methylase Authors: Lunnen, K.D. / Wilson, G.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.6 KB | Display | ![]() |
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PDB format | ![]() | 66.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 5211.398 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: CONTAINS DNA RECOGNITION SEQUENCE OF BGLI | ||||
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#2: Protein | Mass: 34050.793 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: O68557, type II site-specific deoxyribonuclease | ||||
#3: Chemical | ChemComp-CA / #4: Chemical | ChemComp-BME / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.16 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 7-12% PEG 4000, 75-150MM LI2SO4, 100MM TRIS-HCL, 1:2 PROTEIN:DNA MOLAR RATIO, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: CUSTOM-MADE / Detector: CCD / Date: Jun 11, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 17679 / Num. obs: 17679 / % possible obs: 90.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 24.3 Å2 / Rmerge(I) obs: 0.055 / Net I/σ(I): 23.3 |
Reflection shell | Resolution: 2.2→2.25 Å / Redundancy: 0.8 % / Rmerge(I) obs: 0.078 / % possible all: 57.1 |
Reflection | *PLUS |
Reflection shell | *PLUS % possible obs: 57.1 % |
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Processing
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Refinement | Resolution: 2.2→10 Å / Cross valid method: THROUGHOUT / σ(F): 2 Stereochemistry target values: ENGH & HUBER FOR PROTEIN AND PARKINSON ET AL. FOR DNA Details: TO IMPOSE DOUBLE STRANDED BASE-PAIRING RESTRAINTS ON DNA, TWO OLIGONUCLEOTIDE STRANDS WERE INCLUDED IN THE ASYMMETRIC UNIT (RELATED BY CRYSTALLOGRAPHIC TWO- FOLD). THESE WERE GIVEN 0.5 ...Details: TO IMPOSE DOUBLE STRANDED BASE-PAIRING RESTRAINTS ON DNA, TWO OLIGONUCLEOTIDE STRANDS WERE INCLUDED IN THE ASYMMETRIC UNIT (RELATED BY CRYSTALLOGRAPHIC TWO- FOLD). THESE WERE GIVEN 0.5 OCCUPANCY AND WERE REFINED WITH FORCE CONSTANTS FOR VAN DER WAALS AND ELECTROSTATIC INTERACTIONS BETWEEN SYMMETRY RELATED MOLECULES SET TO ZERO. B-DNA SUGAR PUCKER RESTRAINTS WERE IMPOSED ONLY AT THE BEGINNING OF REFINEMENT. THE FOLLOWING SIDE-CHAINS CANNOT BE POSITIONED IN THE ELECTRON DENSITY MAP: GLU7, GLN15, GLU47, GLU102, GLU172, ARG186, THR224, LYS225 AND LYS259
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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