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- PDB-1cq0: SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRU... -

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Basic information

Entry
Database: PDB / ID: 1cq0
TitleSOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '
ComponentsPROTEIN (NEW HYPOTHALAMIC NEUROPEPTIDE/OREXIN-B28)
KeywordsDE NOVO PROTEIN / OBESITY / HUMAN HCRT-2/OX-B / NEUROPEPTIDE / SOLUTION STRUCTURE
Function / homology
Function and homology information


type 1 orexin receptor binding / type 2 orexin receptor binding / neuronal dense core vesicle lumen / negative regulation of transmission of nerve impulse / Orexin and neuropeptides FF and QRFP bind to their respective receptors / positive regulation of transmission of nerve impulse / regulation of neurotransmitter secretion / neuropeptide hormone activity / positive regulation of calcium ion transport / sleep ...type 1 orexin receptor binding / type 2 orexin receptor binding / neuronal dense core vesicle lumen / negative regulation of transmission of nerve impulse / Orexin and neuropeptides FF and QRFP bind to their respective receptors / positive regulation of transmission of nerve impulse / regulation of neurotransmitter secretion / neuropeptide hormone activity / positive regulation of calcium ion transport / sleep / response to alcohol / temperature homeostasis / eating behavior / response to starvation / negative regulation of DNA replication / negative regulation of potassium ion transport / neuropeptide signaling pathway / rough endoplasmic reticulum / excitatory postsynaptic potential / synaptic vesicle / positive regulation of cold-induced thermogenesis / positive regulation of cytosolic calcium ion concentration / phospholipase C-activating G protein-coupled receptor signaling pathway / chemical synaptic transmission / G alpha (q) signalling events / postsynapse / perinuclear region of cytoplasm / extracellular region
Similarity search - Function
Prepro-orexin / Prepro-orexin
Similarity search - Domain/homology
Hypocretin neuropeptide precursor
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / simulated annealing
AuthorsLee, K.-H. / Bang, E.J. / Chae, K.-J. / Lee, D.W. / Lee, W.
CitationJournal: Eur.J.Biochem. / Year: 1999
Title: Solution structure of a new hypothalamic neuropeptide, human hypocretin-2/orexin-B.
Authors: Lee, J.H. / Bang, E. / Chae, K.J. / Kim, J.Y. / Lee, D.W. / Lee, W.
History
DepositionAug 4, 1999Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 10, 2000Provider: repository / Type: Initial release
Revision 1.1Apr 27, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 14, 2018Group: Data collection / Derived calculations
Category: pdbx_nmr_spectrometer / pdbx_struct_assembly ...pdbx_nmr_spectrometer / pdbx_struct_assembly / pdbx_struct_assembly_prop / pdbx_struct_oper_list
Item: _pdbx_nmr_spectrometer.field_strength
Revision 1.4Apr 10, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PROTEIN (NEW HYPOTHALAMIC NEUROPEPTIDE/OREXIN-B28)


Theoretical massNumber of molelcules
Total (without water)2,8941
Polymers2,8941
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area0 Å2
ΔGint0 kcal/mol
Surface area2750 Å2
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 25REM AVERAGE CONFORMATION
Representative

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Components

#1: Protein/peptide PROTEIN (NEW HYPOTHALAMIC NEUROPEPTIDE/OREXIN-B28)


Mass: 2894.336 Da / Num. of mol.: 1 / Fragment: HUMAN HYPOCRETIN-2 / Source method: isolated from a natural source / Details: SYNTHETIC / Source: (natural) Homo sapiens (human) / References: UniProt: O43612

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D-NOESY
121TOCSY
131DQF-COSY

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Sample preparation

DetailsContents: 2mM
Sample conditionsIonic strength: 50 mM Sodium phosphate / pH: 7 / Pressure: 1 bar / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Bruker DRX500 / Manufacturer: Bruker / Model: DRX500 / Field strength: 500 MHz

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Processing

NMR software
NameVersionDeveloperClassification
XPLOR3.85AXEL ET AL.structure solution
XPLOR3.85AXEL ET AL.refinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR ensembleConformer selection criteria: REM AVERAGE CONFORMATION / Conformers calculated total number: 25 / Conformers submitted total number: 1

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