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Open data
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Basic information
Entry | Database: PDB / ID: 1chk | ||||||
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Title | STREPTOMYCES N174 CHITOSANASE PH5.5 298K | ||||||
![]() | CHITOSANASE | ||||||
![]() | HYDROLASE (O-GLYCOSYL) / ANTI-FUNGAL PROTEIN / HYDROLASE / O-GLYCOSYL | ||||||
Function / homology | ![]() chitosanase / chitosanase activity / carbohydrate metabolic process / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Marcotte, E.M. / Robertus, J.D. | ||||||
![]() | ![]() Title: X-ray structure of an anti-fungal chitosanase from streptomyces N174. Authors: Marcotte, E.M. / Monzingo, A.F. / Ernst, S.R. / Brzezinski, R. / Robertus, J.D. #1: ![]() Title: Crystallization of a Chitosanase from Streptomyces N174 Authors: Marcotte, E. / Hart, P.J. / Boucher, I. / Brzezinski, R. / Robertus, J.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.9 KB | Display | ![]() |
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PDB format | ![]() | 77.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.8 KB | Display | ![]() |
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Full document | ![]() | 434 KB | Display | |
Data in XML | ![]() | 19 KB | Display | |
Data in CIF | ![]() | 26.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.593133, -0.777506, -0.208994), Vector: |
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Components
#1: Protein | Mass: 25848.768 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.6 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: pH 5.5 | |||||||||||||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Marcotte, E., (1993) J.Mol.Biol., 232, 995. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Sep 29, 1993 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 21903 / % possible obs: 98 % / Observed criterion σ(I): 0 / Redundancy: 5.5 % / Rmerge(I) obs: 0.0885 |
Reflection | *PLUS Highest resolution: 2.4 Å / % possible obs: 98.7 % / Num. measured all: 121478 |
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Processing
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Refinement | Resolution: 2.4→5 Å / σ(F): 4
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Refinement step | Cycle: LAST / Resolution: 2.4→5 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS % reflection Rfree: 0.236 % / Rfactor obs: 0.1809 / Rfactor Rfree: 0.1748 / Rfactor Rwork: 0.1809 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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