+Open data
-Basic information
Entry | Database: PDB / ID: 1cgn | ||||||||||||
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Title | CYTOCHROME C' | ||||||||||||
Components | CYTOCHROME C | ||||||||||||
Keywords | ELECTRON TRANSPORT (CYTOCHROME) | ||||||||||||
Function / homology | Function and homology information electron transfer activity / periplasmic space / iron ion binding / heme binding Similarity search - Function | ||||||||||||
Biological species | Achromobacter xylosoxidans (bacteria) | ||||||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.15 Å | ||||||||||||
Authors | Dobbs, A.J. / Faber, H.R. / Anderson, B.F. / Baker, E.N. | ||||||||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1996 Title: Three-dimensional structure of cytochrome c' from two Alcaligenes species and the implications for four-helix bundle structures. Authors: Dobbs, A.J. / Anderson, B.F. / Faber, H.R. / Baker, E.N. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Atomic Structure of a Cytochrome C' with an Unusual Ligand-Controlled Dimer Dissociation at 1.8 Angstroms Resolution Authors: Ren, Z. / Meyer, T.E. / Mcree, D.E. #2: Journal: J.Biochem.(Tokyo) / Year: 1992 Title: Three-Dimensional Structure of Ferricytochrome C' from Rhodospirillum Rubrum at 2.8 Angstroms Resolution Authors: Yasui, M. / Harada, S. / Kai, Y. / Kasai, N. / Kusunoki, M. / Matsura, Y. #3: Journal: J.Mol.Biol. / Year: 1985 Title: Structure of Ferricytochrome C' from Rhodospirillum Molischianum at 1.67 Angstroms Authors: Finzel, B.C. / Weber, P.C. / Hardman, K.D. / Salemme, F.R. #4: Journal: J.Mol.Biol. / Year: 1981 Title: Crystallographic Structure of Rhodospirillum Molischianum Ferricytochrome C' at 2.5 Angstroms Resolution Authors: Weber, P.C. / Howard, A. / Xoung, N.H. / Salemme, F.R. #5: Journal: Biochem.J. / Year: 1973 Title: The Amino Acid Sequence of Cytochrome C' from Alcaligenes Sp. N.C.I.B. 11015 Authors: Ambler, R.P. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cgn.cif.gz | 35 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cgn.ent.gz | 26.7 KB | Display | PDB format |
PDBx/mmJSON format | 1cgn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cg/1cgn ftp://data.pdbj.org/pub/pdb/validation_reports/cg/1cgn | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | SYMMETRY THE CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS PRESENTED BELOW GENERATE THE SUBUNITS OF THE POLYMERIC MOLECULE. APPLIED TO RESIDUES: PCA 1 .. HEM 128 SYMMETRY OPERATION TO GENERATE THE SECOND MOLECULE OF THE DIMERIC PARTICLE SYMMETRY1 1 -1.000000 0.000000 0.000000 0.00000 SYMMETRY2 1 0.000000 1.000000 0.000000 0.00000 SYMMETRY3 1 0.000000 0.000000 -1.000000 90.20000 |
-Components
#1: Protein | Mass: 13575.380 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Achromobacter xylosoxidans (bacteria) / References: UniProt: P00138 |
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#2: Chemical | ChemComp-HEC / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 56.96 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 37 ℃ / pH: 6 / Method: vapor diffusion / Details: Norris, G. E., (1979) J.Mol.Biol., 135, 309. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Mar 20, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Redundancy: 7.4 % / Rmerge(I) obs: 0.032 |
Reflection | *PLUS Highest resolution: 2.15 Å / Num. obs: 8220 / % possible obs: 90 % / Observed criterion σ(I): 0 / Num. measured all: 60526 / Rmerge(I) obs: 0.032 |
-Processing
Software |
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Refinement | Resolution: 2.15→20 Å / σ(F): 0 /
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Refinement step | Cycle: LAST / Resolution: 2.15→20 Å
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||
Refinement | *PLUS | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS Biso mean: 22.9 Å2 | ||||||||||||
Refine LS restraints | *PLUS
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