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Yorodumi- PDB-1cf2: THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYD... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1cf2 | ||||||
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Title | THREE-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEON METHANOTHERMUS FERVIDUS | ||||||
Components | PROTEIN (GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE) | ||||||
Keywords | OXIDOREDUCTASE / OXYDOREDUCTASE | ||||||
Function / homology | Function and homology information glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) / glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity / 4-hydroxy-tetrahydrodipicolinate reductase / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity / lysine biosynthetic process via diaminopimelate / glycolytic process / NAD binding / NADP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Methanothermus fervidus (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Charron, C. / Talfournier, F. / Isuppov, M.N. / Branlant, G. / Littlechild, J.A. / Vitoux, B. / Aubry, A. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: Crystallization and preliminary X-ray diffraction studies of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus. Authors: Charron, C. / Talfournier, F. / Isupov, M.N. / Branlant, G. / Littlechild, J.A. / Vitoux, B. / Aubry, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cf2.cif.gz | 288.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cf2.ent.gz | 235.9 KB | Display | PDB format |
PDBx/mmJSON format | 1cf2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cf/1cf2 ftp://data.pdbj.org/pub/pdb/validation_reports/cf/1cf2 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 37454.793 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanothermus fervidus (archaea) / Production host: Escherichia coli (E. coli) / Strain (production host): HB101 References: UniProt: P10618, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) #2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-NAP / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.01 % |
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Crystal grow | pH: 6.5 / Details: pH 6.50 |
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Jancarik, J., (1991) J. Appl. Crystallogr., 24, 409. |
Components of the solutions | *PLUS Conc.: 10 mg/ml / Common name: protein |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM02 / Wavelength: 0.987 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.987 Å / Relative weight: 1 |
Reflection | Resolution: 2.11→10 Å / Num. obs: 82965 / % possible obs: 91.7 % / Observed criterion σ(I): 2 / Redundancy: 5 % / Rsym value: 5.6 / Net I/σ(I): 9.1 |
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 10 Å / % possible obs: 91.7 % / Observed criterion σ(I): 2 / Redundancy: 5 % / Num. measured all: 415252 / Rmerge(I) obs: 0.056 |
Reflection shell | *PLUS Highest resolution: 2.11 Å / Lowest resolution: 2.17 Å / % possible obs: 69.3 % / Rmerge(I) obs: 0.151 / Mean I/σ(I) obs: 4 |
-Processing
Software | Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.1→10 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.1→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 10 Å / Rfactor obs: 0.194 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |