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- PDB-1c7q: THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTIL... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1c7q | ||||||
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Title | THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTILITY FACTOR/NEUROLEUKIN COMPLEXED WITH ITS CARBOHYDRATE PHOSPHATE INHIBITORS AND ITS SUBSTRATE RECOGNITION MECHANISM | ||||||
![]() | PHOSPHOGLUCOSE ISOMERASE | ||||||
![]() | ISOMERASE / PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTILITY FACTOR/ NEUROLEUKIN | ||||||
Function / homology | ![]() glucose-6-phosphate isomerase / glucose-6-phosphate isomerase activity / glucose 6-phosphate metabolic process / carbohydrate derivative binding / monosaccharide binding / gluconeogenesis / glycolytic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chou, C.-C. / Meng, M. / Sun, Y.-J. / Hsiao, C.-D. | ||||||
![]() | ![]() Title: The crystal structure of phosphoglucose isomerase/autocrine motility factor/neuroleukin complexed with its carbohydrate phosphate inhibitors suggests its substrate/receptor recognition. Authors: Chou, C.C. / Sun, Y.J. / Meng, M. / Hsiao, C.D. #1: ![]() Title: The Crystal Structure of a Multifunctional Protein: Phosphoglucose Isomerase/Autocrine Motility Factor/ Neuroleukin Authors: Sun, Y.-J. / Chou, C.-C. / Chen, W.-S. / Wu, R.-T. / Meng, M. / Hsiao, C.-D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 102.8 KB | Display | ![]() |
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PDB format | ![]() | 78 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 803.9 KB | Display | ![]() |
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Full document | ![]() | 812.7 KB | Display | |
Data in XML | ![]() | 19.4 KB | Display | |
Data in CIF | ![]() | 27.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1b0zC ![]() 1c7rC ![]() 2pgiS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50202.758 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Cellular location: CYTOPLASM / Plasmid: PFDI22 / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-BE1 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 57.88 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: 0.1M HEPES PH 7.5, 0.8M K, NA TARTRATE | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Aug 1, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→34.65 Å / Num. obs: 30710 / % possible obs: 98.6 % / Observed criterion σ(I): 2 / Redundancy: 3.57 % / Biso Wilson estimate: 13.4 Å2 / Rmerge(I) obs: 0.075 / Net I/σ(I): 15 |
Reflection shell | Resolution: 2.3→2.44 Å / Redundancy: 3.49 % / Rmerge(I) obs: 0.316 / Mean I/σ(I) obs: 3.2 / % possible all: 93.7 |
Reflection | *PLUS Num. measured all: 109736 |
Reflection shell | *PLUS % possible obs: 98 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2PGI Resolution: 2.3→8 Å / Rfactor Rfree error: 0.005 / Cross valid method: THROUGHOUT / σ(F): 2
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Displacement parameters | Biso mean: 21.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.44 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 6
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 8 Å / σ(F): 2 / % reflection Rfree: 9.9 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 21.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2.3 Å / Rfactor Rfree: 0.281 / % reflection Rfree: 10.9 % / Rfactor Rwork: 0.217 |