分子量: 4471.667 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: CLOSED BY A UUCG TETRALOOP; COBALT (III) HEXAMMINE BINDS AT THE MAJOR GROOVE OF TWO TANDEM G-U WOBBLE PAIRS IN 5'-GU-3' ORIENTATION 由来: (組換発現) Tetrahymena thermophila (真核生物) 解説: SYNTHESIZED ENZYMATICALLY IN-VITRO USING T7 RNA POLYMERASE
Text: THE STRUCTURE WAS DETERMINED USING A NON-ISOTOPICALLY LABELLED RNA SAMPLE. ALL 13C EXPERIMENTS WERE DONE USING NATURAL ABUNDANCE. COBALT (III) HEXAMMINE WAS LOCATED USING DOCKING WITH 7 ...Text: THE STRUCTURE WAS DETERMINED USING A NON-ISOTOPICALLY LABELLED RNA SAMPLE. ALL 13C EXPERIMENTS WERE DONE USING NATURAL ABUNDANCE. COBALT (III) HEXAMMINE WAS LOCATED USING DOCKING WITH 7 INTERMOLECULAR NOES OBSERVED IN 2D WATER NOESY EXPERIMENTS.
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試料調製
詳細
内容: PHOSPHATE BUFFER, EDTA
試料状態
イオン強度: 100 mM NACL / pH: 6.4 / 圧: 1 atm / 温度: 298 K
結晶化
*PLUS
手法: その他 / 詳細: NMR
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker AMX600
Bruker
AMX600
500
1
Bruker DRX500
Bruker
DRX500
600
2
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解析
NMR software
名称
バージョン
開発者
分類
X-PLOR
3.1
BRUNGER
精密化
FELIX95
構造決定
X-PLOR
3.1
構造決定
精密化
手法: RESTRAINED MOLECULAR DYNAMICS / ソフトェア番号: 1 詳細: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE
NMRアンサンブル
コンフォーマー選択の基準: NOE ENERGY (LEAST RESTRAINT VIOLATIONS), DIHEDRAL ENERGY (LEAST RESTRAINT VIOLATIONS), TOTAL ENERGY 計算したコンフォーマーの数: 40 / 登録したコンフォーマーの数: 19