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Open data
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Basic information
Entry | Database: PDB / ID: 1c0b | ||||||
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Title | BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 2.5 DAYS | ||||||
![]() | RIBONUCLEASE A | ||||||
![]() | HYDROLASE / HYDROLASE (PHOSPHORIC DIESTER / RNA) / DESICCATED / DRY / DEHYDRATED | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bell, J.A. | ||||||
![]() | ![]() Title: X-ray crystal structures of a severely desiccated protein Authors: Bell, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 32.5 KB | Display | ![]() |
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PDB format | ![]() | 24.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 429.9 KB | Display | ![]() |
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Full document | ![]() | 447.2 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 11.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 14062.729 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 295 K / Method: small tubes / pH: 4.5 Details: 58% methanol (v/v), pH 4.5, SMALL TUBES, temperature 295K |
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PRINCETON 2K / Detector: CCD / Date: Sep 25, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→99 Å / Num. all: 5880 / Num. obs: 5532 / % possible obs: 91 % / Observed criterion σ(I): 1 / Redundancy: 6.5 % / Biso Wilson estimate: 38.7 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 33.2 |
Reflection shell | Resolution: 1.9→2.1 Å / Num. unique all: 1229 / % possible all: 80 |
Reflection | *PLUS Lowest resolution: 9999 Å / Num. measured all: 38053 / Rmerge(I) obs: 0.06 |
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Processing
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Refinement | Resolution: 1.9→20 Å / Cross valid method: THROUGHOUT / σ(I): 1 / Stereochemistry target values: Protgeo Details: Final five cycles of refinement included all observed data
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5E / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 20 Å / % reflection Rfree: 10 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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