Mass: 2581.601 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: THE RNA:RNA-DNA CHIMERIC HYBRID DUPLEX WAS CHEMICALLY SYNTHESIZED FROM THE HUMAN IMMUNODIFICIENCY VIRUS TYPE 1 (HIV-1). THE DUPLEX IS FORMED DURING THE INITIATION OF (-)DNA STRAND SYNTHESIS IN HIV-1 REPLICATION.
#2: DNA/RNA hybrid
DNA/RNA (5'-R(*GP*CP*CP*A)-D(P*CP*TP*GP*C)-3')
Mass: 2451.580 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: CHIMERIC HYBRID RNA/RNA-DNA DUPLEX
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Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
TOCSY
1
3
1
DQF-COSY
1
4
1
E H-P HETCOR
1
5
1
H-C COSY
NMR details
Text: THE 20 STRUCTURES ARE ENERGY-MINIMIZED WITH THE AMBER FORCE FIELD USING THE PROGRAM OPAL
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Sample preparation
Sample conditions
pH: 7.0 / Pressure: 1 atm / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR
-
NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AMX
Bruker
AMX
500
1
Bruker AMX
Bruker
AMX
600
2
Varian UNITY
Varian
UNITY
750
3
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Processing
NMR software
Name
Developer
Classification
DYANA
GUNTERT,WUTHRICH
refinement
DYANA
structuresolution
Refinement
Method: distance geometry / Software ordinal: 1 Details: REFINEMENT DETAILS ARE DESCRIBED IN THE JRNL CITATION ABOVE
NMR ensemble
Conformer selection criteria: DYANA TARGET FUNCTION / Conformers calculated total number: 100 / Conformers submitted total number: 20
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