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Yorodumi- PDB-1byh: MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1byh | |||||||||
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Title | MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE | |||||||||
Components | HYBRID | |||||||||
Keywords | HYDROLASE | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | synthetic construct (others) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | |||||||||
Authors | Keitel, T. / Heinemann, U. | |||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1993 Title: Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase. Authors: Keitel, T. / Simon, O. / Borriss, R. / Heinemann, U. #1: Journal: MGG,Mol.Gen.Genet. / Year: 1991 Title: Hybrid Bacillus (1-3,1-4)-Beta-Glucanase: Engineering Thermostable Enzymes by Construction of Hybrid Genes Authors: Olsen, O. / Borriss, R. / Simon, O. / Thomsen, K. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1byh.cif.gz | 54.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1byh.ent.gz | 41 KB | Display | PDB format |
PDBx/mmJSON format | 1byh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1byh_validation.pdf.gz | 749.7 KB | Display | wwPDB validaton report |
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Full document | 1byh_full_validation.pdf.gz | 765.6 KB | Display | |
Data in XML | 1byh_validation.xml.gz | 13.2 KB | Display | |
Data in CIF | 1byh_validation.cif.gz | 17.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/by/1byh ftp://data.pdbj.org/pub/pdb/validation_reports/by/1byh | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE 201 IS A CIS PROLINE. |
-Components
#1: Protein | Mass: 23935.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / References: EMBL: Z25878, UniProt: P23904*PLUS, licheninase |
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#2: Polysaccharide | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose / beta-cellobiose |
#3: Chemical | ChemComp-CA / |
#4: Chemical | ChemComp-NBU / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.65 % |
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Conc.: 12-16 %(w/v) / Common name: PEG6000 |
-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.6 Å / Num. obs: 8984 / Num. measured all: 14705 / Rmerge(I) obs: 0.037 |
-Processing
Software |
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Refinement | Resolution: 2.8→8 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 8 Å / Num. reflection obs: 4759 / σ(F): 1 / Rfactor obs: 0.168 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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