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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1bx2 | ||||||
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タイトル | CRYSTAL STRUCTURE OF HLA-DR2 (DRA*0101,DRB1*1501) COMPLEXED WITH A PEPTIDE FROM HUMAN MYELIN BASIC PROTEIN | ||||||
![]() | (PROTEIN (HLA-DR2)) x 3 | ||||||
![]() | IMMUNE SYSTEM / HLA-DR2 / MYELIN BASIC PROTEIN / MULTIPLE SCLEROSIS / AUTOIMMUNITY | ||||||
機能・相同性 | ![]() compact myelin / structural constituent of myelin sheath / positive regulation of metalloendopeptidase activity / internode region of axon / axon ensheathment / negative regulation of heterotypic cell-cell adhesion / regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II ...compact myelin / structural constituent of myelin sheath / positive regulation of metalloendopeptidase activity / internode region of axon / axon ensheathment / negative regulation of heterotypic cell-cell adhesion / regulation of interleukin-4 production / regulation of interleukin-10 production / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / EGR2 and SOX10-mediated initiation of Schwann cell myelination / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of T cell mediated immune response to tumor cell / positive regulation of kinase activity / positive regulation of memory T cell differentiation / membrane organization / CD4 receptor binding / positive regulation of monocyte differentiation / inflammatory response to antigenic stimulus / positive regulation of chemokine (C-X-C motif) ligand 2 production / intermediate filament / T-helper 1 type immune response / transport vesicle membrane / maintenance of blood-brain barrier / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / polysaccharide binding / negative regulation of type II interferon production / humoral immune response / Generation of second messenger molecules / positive regulation of insulin secretion involved in cellular response to glucose stimulus / macrophage differentiation / immunological synapse / Co-inhibition by PD-1 / epidermis development / detection of bacterium / T cell receptor binding / negative regulation of T cell proliferation / myelination / substantia nigra development / MHC class II antigen presentation / central nervous system development / trans-Golgi network membrane / cell periphery / lumenal side of endoplasmic reticulum membrane / protein tetramerization / peptide antigen assembly with MHC class II protein complex / negative regulation of inflammatory response to antigenic stimulus / sensory perception of sound / MHC class II protein complex / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / peptide antigen binding / structural constituent of cytoskeleton / positive regulation of T cell mediated cytotoxicity / positive regulation of interleukin-6 production / positive regulation of T cell activation / cognition / response to toxic substance / Interferon gamma signaling / MHC class II protein complex binding / endocytic vesicle membrane / late endosome membrane / MAPK cascade / Downstream TCR signaling / myelin sheath / T cell receptor signaling pathway / positive regulation of protein phosphorylation / protease binding / early endosome membrane / chemical synaptic transmission / adaptive immune response / positive regulation of canonical NF-kappaB signal transduction / positive regulation of viral entry into host cell / lysosome / positive regulation of ERK1 and ERK2 cascade / positive regulation of MAPK cascade / calmodulin binding / immune response / external side of plasma membrane / Golgi membrane / lysosomal membrane / neuronal cell body / synapse / lipid binding / positive regulation of DNA-templated transcription / cell surface / signal transduction / protein-containing complex / extracellular space / extracellular exosome / nucleus / membrane 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Smith, K.J. / Pyrdol, J. / Gauthier, L. / Wiley, D.C. / Wucherpfennig, K. | ||||||
![]() | ![]() タイトル: Crystal structure of HLA-DR2 (DRA*0101, DRB1*1501) complexed with a peptide from human myelin basic protein. 著者: Smith, K.J. / Pyrdol, J. / Gauthier, L. / Wiley, D.C. / Wucherpfennig, K.W. #1: ![]() タイトル: Molecular Mimicry in T Cell Mediated Autoimmunity:Viral Peptides Activate Human T Cell Clones Specific for Myelin Basic Protein 著者: Wucherpfennig, K. / Strominger, J.L. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 166 KB | 表示 | ![]() |
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PDB形式 | ![]() | 132.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 425.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 448 KB | 表示 | |
XML形式データ | ![]() | 18.7 KB | 表示 | |
CIF形式データ | ![]() | 27.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 1dlhS S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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2 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 20971.670 Da / 分子数: 2 / 断片: EXTRACELLULAR DOMAINS ALPHA 1, ALPHA 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: タンパク質 | 分子量: 22316.930 Da / 分子数: 2 / 断片: EXTRACELLULAR DOMAINS BETA 1, BETA 2 / 由来タイプ: 天然 / 由来: (天然) ![]() #3: タンパク質・ペプチド | 分子量: 1800.086 Da / 分子数: 2 / 断片: PEPTIDE FROM HUMAN MYELIN BASIC PROTEIN / 由来タイプ: 天然 詳細: MYELIN BASIC PROTEIN PEPTIDE WAS COVALENTLY LINKED TO THE N-TERMINUS OF THE HLA-DR2 BETA CHAIN 由来: (天然) ![]() #4: 糖 | #5: 水 | ChemComp-HOH / | Has protein modification | Y | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.2 Å3/Da / 溶媒含有率: 62 % | |||||||||||||||||||||||||
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結晶化 | pH: 3.5 / 詳細: 15-18% PEG6000 100MM GLYCINE PH3.5 | |||||||||||||||||||||||||
結晶化 | *PLUS 温度: 18 ℃ / pH: 7.5 / 手法: unknown | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC / 検出器: CCD / 日付: 1998年3月18日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.92 Å / 相対比: 1 |
反射 | 解像度: 2.6→20 Å / Num. obs: 42589 / % possible obs: 88.3 % / Observed criterion σ(I): -3 / 冗長度: 6 % / Biso Wilson estimate: 37.6 Å2 / Rmerge(I) obs: 0.088 / Net I/σ(I): 23 |
反射 シェル | 解像度: 2.49→2.6 Å / Rmerge(I) obs: 0.27 / Mean I/σ(I) obs: 3.9 / % possible all: 83.1 |
反射 | *PLUS Num. measured all: 263333 |
反射 シェル | *PLUS % possible obs: 83.1 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 1DLH 解像度: 2.6→20 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 2
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原子変位パラメータ | Biso mean: 35.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.6→20 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.6→2.72 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 8
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Xplor file |
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