+Open data
-Basic information
Entry | Database: PDB / ID: 1bwt | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2] | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / ECORI RECOGNITION SITE | Function / homology | DNA | Function and homology information Method | SOLUTION NMR / simulated annealing | Authors | Aramini, J.M. / Mujeeb, A. / Germann, M.W. | Citation | Journal: Nucleic Acids Res. / Year: 1998 | Title: NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control. Authors: Aramini, J.M. / Mujeeb, A. / Germann, M.W. History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1bwt.cif.gz | 20.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1bwt.ent.gz | 13.1 KB | Display | PDB format |
PDBx/mmJSON format | 1bwt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bwt_validation.pdf.gz | 235.7 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1bwt_full_validation.pdf.gz | 235.5 KB | Display | |
Data in XML | 1bwt_validation.xml.gz | 1.7 KB | Display | |
Data in CIF | 1bwt_validation.cif.gz | 1.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bw/1bwt ftp://data.pdbj.org/pub/pdb/validation_reports/bw/1bwt | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: DNA chain | Mass: 3045.005 Da / Num. of mol.: 2 / Source method: obtained synthetically |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||
NMR details | Text: MEAN STRUCTURE. THE STRUCTURE WAS DETERMINED USING DISTANCE AND TORSION ANGLE RESTRAINTS OBTAINED FROM HOMONUCLEAR NOESY (125, 250 MS MIXING TIMES) AND DQF- COSY EXPERIMENTS. |
-Sample preparation
Sample conditions | Ionic strength: 50 mM NACL, 10 mM NAPHOSPHATE, 0.1 mM EDTA / pH: 6.5 / Pressure: 1 atm / Temperature: 303 K |
---|---|
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||||||
NMR ensemble | Conformers calculated total number: 9 / Conformers submitted total number: 1 |