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Open data
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Basic information
Entry | Database: PDB / ID: 1bsw | ||||||
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Title | ACUTOLYSIN A FROM SNAKE VENOM OF AGKISTRODON ACUTUS AT PH 7.5 | ||||||
![]() | PROTEIN (ACUTOLYSIN A) | ||||||
![]() | METAL BINDING PROTEIN / METALLOPROTEINASE / SNAKE VENOM / MMP | ||||||
Function / homology | ![]() envenomation resulting in hemorrhagic damage in another organism / Hydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases / metalloendopeptidase activity / toxin activity / calcium ion binding / proteolysis / zinc ion binding / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gong, W. / Zhu, X. / Liu, S. / Teng, M. / Niu, L. | ||||||
![]() | ![]() Title: Crystal structures of acutolysin A, a three-disulfide hemorrhagic zinc metalloproteinase from the snake venom of Agkistrodon acutus. Authors: Gong, W. / Zhu, X. / Liu, S. / Teng, M. / Niu, L. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Studies of Haehorrhagin I from the Snake Venom of Agkistrodon Acutus Authors: Gong, W. / Zhu, X. / Niu, L. / Teng, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.1 KB | Display | ![]() |
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PDB format | ![]() | 37.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 365.7 KB | Display | ![]() |
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Full document | ![]() | 366.5 KB | Display | |
Data in XML | ![]() | 5.4 KB | Display | |
Data in CIF | ![]() | 8.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1budC ![]() 1iagS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21850.730 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.48 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: SEALED TUBE / Type: OTHER / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromator: GRAPHITE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→10 Å / Num. obs: 12809 / % possible obs: 88.3 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.083 |
Reflection shell | Resolution: 1.95→2.1 Å / Rmerge(I) obs: 0.283 / % possible all: 76.6 |
Reflection | *PLUS Observed criterion σ(F): 1 |
Reflection shell | *PLUS % possible obs: 76.6 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1IAG Resolution: 1.95→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 17.85 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(F): 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.6 |