+
Open data
-
Basic information
Entry | Database: PDB / ID: 1bs0 | ||||||
---|---|---|---|---|---|---|---|
Title | PLP-DEPENDENT ACYL-COA SYNTHASE | ||||||
![]() | PROTEIN (8-AMINO-7-OXONANOATE SYNTHASE) | ||||||
![]() | TRANSFERASE / PLP-DEPENDENT ACYL-COA SYNTHASE / BIOTIN BIOSYNTHESIS / 8-AMINO-7-OXONANOATE SYNTHASE / 8-AMINO-7-KETOPELARGONATE SYNTHASE | ||||||
Function / homology | ![]() 8-amino-7-oxononanoate synthase / 8-amino-7-oxononanoate synthase activity / biotin biosynthetic process / pyridoxal phosphate binding / protein homodimerization activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Alexeev, D. / Alexeeva, M. / Baxter, R.L. / Campopiano, D.J. / Webster, S.P. / Sawyer, L. | ||||||
![]() | ![]() Title: The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme. Authors: Alexeev, D. / Alexeeva, M. / Baxter, R.L. / Campopiano, D.J. / Webster, S.P. / Sawyer, L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 99.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 75.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 383.3 KB | Display | |
Data in XML | ![]() | 9.7 KB | Display | |
Data in CIF | ![]() | 17.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||||||||
2 | ![]()
| |||||||||||||||
Unit cell |
| |||||||||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 41641.172 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P12998, 8-amino-7-oxononanoate synthase | ||
---|---|---|---|
#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.3 % | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 7.9 Details: PROTEIN WAS CRYSTALLIZED FROM 0.2M AMMONIUM SULPHATE, 200MM BIS-TRIS,, pH 7.9 | ||||||||||||||||||||
Crystal | *PLUS Density % sol: 46 % | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 15, 1996 / Details: BENT MIRROR |
Radiation | Monochromator: SUPPER DOUBLE MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.911 Å / Relative weight: 1 |
Reflection | Resolution: 1.64→15 Å / Num. obs: 46252 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 14.3 % / Rmerge(I) obs: 0.084 / Rsym value: 0.084 / Net I/σ(I): 31.3 |
Reflection shell | Resolution: 1.65→1.68 Å / Redundancy: 8.8 % / Rmerge(I) obs: 0.369 / Mean I/σ(I) obs: 3.3 / Rsym value: 0.369 / % possible all: 100 |
Reflection shell | *PLUS % possible obs: 99.6 % |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Details: SOME FURTHER REFINEMENT HAS BEEN PERFORMED SINCE THE PAPER WAS SUBMITTED TO J.MOL.BIOL.
| |||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973) 201-228 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 2 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 3359.1 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→10 Å
| |||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||
Software | *PLUS Name: SHELXL-97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 10 Å / Rfactor Rwork: 0.178 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |