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- PDB-1brg: CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1brg | ||||||
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Title | CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE | ||||||
![]() | BARNASE | ||||||
![]() | ENDONUCLEASE | ||||||
Function / homology | ![]() Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters / RNA endonuclease activity / RNA binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Chen, Y.W. / Fersht, A.R. / Henrick, K. | ||||||
![]() | ![]() Title: Crystallographic analysis of Phe-->Leu substitution in the hydrophobic core of barnase. Authors: Chen, Y.W. / Fersht, A.R. / Henrick, K. #1: ![]() Title: Contribution of Hydrophobic Interactions to Protein Stability Authors: Kellis Junior, J.T. / Nyberg, K. / Sali, D. / Fersht, A.R. #2: ![]() Title: Molecular Structures of a New Family of Ribonucleases Authors: Mauguen, Y. / Hartley, R.W. / Dodson, E.J. / Dodson, G.G. / Bricogne, G. / Chothia, C. / Jack, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.7 KB | Display | ![]() |
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PDB format | ![]() | 59.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.9 KB | Display | ![]() |
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Full document | ![]() | 454.9 KB | Display | |
Data in XML | ![]() | 19 KB | Display | |
Data in CIF | ![]() | 26.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12165.499 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: P00648, Hydrolases; Acting on ester bonds; Endoribonucleases producing 3'-phosphomonoesters #2: Chemical | ChemComp-ZN / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.01 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Mauguen, Y., (1982) Nature, 297, 162. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 6 Å / Num. obs: 15097 / % possible obs: 94 % / Redundancy: 1.5 % / Num. measured all: 26504 / Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.26 Å / % possible obs: 77.1 % / Redundancy: 1.4 % / Rmerge(I) obs: 0.053 |
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Processing
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Refinement | Resolution: 2.2→6 Å / σ(F): 3 /
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Refinement step | Cycle: LAST / Resolution: 2.2→6 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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