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Open data
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Basic information
Entry | Database: PDB / ID: 1blx | ||||||
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Title | P19INK4D/CDK6 COMPLEX | ||||||
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![]() | COMPLEX (INHIBITOR PROTEIN/KINASE) / INHIBITOR PROTEIN / CYCLIN-DEPENDENT KINASE / CELL CYCLE CONTROL / ALPHA/BETA / COMPLEX (INHIBITOR PROTEIN-KINASE) / COMPLEX (INHIBITOR PROTEIN-KINASE) complex | ||||||
Function / homology | ![]() cyclin D2-CDK6 complex / cyclin D2-CDK4 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / autophagic cell death / lateral ventricle development / negative regulation of cell cycle G1/S phase transition ...cyclin D2-CDK6 complex / cyclin D2-CDK4 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / autophagic cell death / lateral ventricle development / negative regulation of cell cycle G1/S phase transition / negative regulation of myeloid cell differentiation / type B pancreatic cell development / negative regulation of monocyte differentiation / astrocyte development / dentate gyrus development / negative regulation of phosphorylation / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage / cyclin-dependent protein serine/threonine kinase inhibitor activity / response to vitamin D / gliogenesis / regulation of cell motility / Regulation of RUNX1 Expression and Activity / regulation of hematopoietic stem cell differentiation / positive regulation of cell-matrix adhesion / generation of neurons / DNA synthesis involved in DNA repair / regulation of G1/S transition of mitotic cell cycle / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / negative regulation of cellular senescence / negative regulation of cell differentiation / negative regulation of cell cycle / cyclin-dependent protein kinase holoenzyme complex / hematopoietic stem cell differentiation / negative regulation of osteoblast differentiation / cyclin-dependent kinase / cyclin-dependent protein serine/threonine kinase activity / response to UV / response to retinoic acid / Notch signaling pathway / ruffle / regulation of G2/M transition of mitotic cell cycle / cyclin binding / : / sensory perception of sound / response to virus / regulation of erythrocyte differentiation / Oncogene Induced Senescence / negative regulation of cell growth / G1/S transition of mitotic cell cycle / Cyclin D associated events in G1 / negative regulation of epithelial cell proliferation / positive regulation of fibroblast proliferation / T cell differentiation in thymus / Senescence-Associated Secretory Phenotype (SASP) / regulation of gene expression / Oxidative Stress Induced Senescence / regulation of cell cycle / cell cycle / negative regulation of cell population proliferation / cell division / protein phosphorylation / protein serine kinase activity / centrosome / positive regulation of gene expression / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / nucleoplasm / ATP binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brotherton, D.H. / Dhanaraj, V. / Wick, S. / Brizuela, L. / Domaille, P.J. / Volyanik, E. / Xu, X. / Parisini, E. / Smith, B.O. / Archer, S.J. ...Brotherton, D.H. / Dhanaraj, V. / Wick, S. / Brizuela, L. / Domaille, P.J. / Volyanik, E. / Xu, X. / Parisini, E. / Smith, B.O. / Archer, S.J. / Serrano, M. / Brenner, S.L. / Blundell, T.L. / Laue, E.D. | ||||||
![]() | ![]() Title: Crystal structure of the complex of the cyclin D-dependent kinase Cdk6 bound to the cell-cycle inhibitor p19INK4d. Authors: Brotherton, D.H. / Dhanaraj, V. / Wick, S. / Brizuela, L. / Domaille, P.J. / Volyanik, E. / Xu, X. / Parisini, E. / Smith, B.O. / Archer, S.J. / Serrano, M. / Brenner, S.L. / Blundell, T.L. / Laue, E.D. #1: ![]() Title: Erratum. Crystal Structure of the Complex of the Cyclin D-Dependent Kinase Cdk6 Bound to the Cell-Cycle Inhibitor P19Ink4D Authors: Brotherton, D.H. / Dhanaraj, V. / Wick, S. / Brizuela, L. / Domaille, P.J. / Volyanik, E. / Xu, X. / Parisini, E. / Smith, B.O. / Archer, S.J. / Serrano, M. / Brenner, S.L. / Blundell, T.L. / Laue, E.D. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.5 KB | Display | ![]() |
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PDB format | ![]() | 83.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377.2 KB | Display | ![]() |
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Full document | ![]() | 394.6 KB | Display | |
Data in XML | ![]() | 12.5 KB | Display | |
Data in CIF | ![]() | 20.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 36987.328 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Protein | Mass: 17832.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.27 % | ||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 279 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 1, 1998 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9475 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→18.54 Å / Num. obs: 41965 / % possible obs: 98.5 % / Redundancy: 4 % / Rmerge(I) obs: 0.054 / Rsym value: 0.054 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.213 / Mean I/σ(I) obs: 1.9 / Rsym value: 0.327 / % possible all: 97.7 |
Reflection | *PLUS Num. measured all: 166218 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 1HCK AND 1AP7 Resolution: 1.9→18.54 Å / Cross valid method: FREE R / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.9→18.54 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.2 | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS
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