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Open data
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Basic information
Entry | Database: PDB / ID: 1bf6 | ||||||
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Title | PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI | ||||||
![]() | PHOSPHOTRIESTERASE HOMOLOGY PROTEIN | ||||||
![]() | PHOSPHOTRIESTERASE / HYPOTHETICAL PROTEIN | ||||||
Function / homology | ![]() catabolic process / hydrolase activity, acting on ester bonds / Hydrolases; Acting on ester bonds / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Buchbinder, J.L. / Stephenson, R.C. / Scanlan, T.S. / Fletterick, R.J. | ||||||
![]() | ![]() Title: Biochemical characterization and crystallographic structure of an Escherichia coli protein from the phosphotriesterase gene family. Authors: Buchbinder, J.L. / Stephenson, R.C. / Dresser, M.J. / Pitera, J.W. / Scanlan, T.S. / Fletterick, R.J. #1: ![]() Title: Erratum. Biochemical Characterization and Crystallographic Structure of an Escherichia Coli Protein from the Phosphotriesterase Gene Family Authors: Buchbinder, J.L. / Stephenson, R.C. / Dresser, M.J. / Pitera, J.W. / Scanlan, T.S. / Fletterick, R.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 131.6 KB | Display | ![]() |
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PDB format | ![]() | 101.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.999993, -0.000858, -0.000305), Vector: |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 32820.184 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 5 types, 296 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / #3: Chemical | #4: Chemical | ChemComp-MPD / ( | #5: Chemical | ChemComp-GOL / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 51.24 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 105 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Oct 1, 1996 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→50 Å / Num. obs: 69475 / % possible obs: 96 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 11.7 Å2 / Rmerge(I) obs: 0.075 / Rsym value: 0.08 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 1.7→1.8 Å / Redundancy: 2 % / Rmerge(I) obs: 0.24 / Mean I/σ(I) obs: 3 / Rsym value: 0.3 / % possible all: 78 |
Reflection | *PLUS Num. measured all: 286468 |
Reflection shell | *PLUS % possible obs: 78 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 16.42 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.7→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.78 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 8
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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