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- PDB-1bbj: CRYSTAL STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY BIND... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1bbj | |||||||||
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Title | CRYSTAL STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY BINDING TUMOUR CELLS | |||||||||
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![]() | IMMUNOGLOBULIN | |||||||||
Function / homology | ![]() IgG immunoglobulin complex / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / immunoglobulin complex, circulating / immunoglobulin receptor binding / FCGR3A-mediated IL10 synthesis / complement activation, classical pathway / Regulation of Complement cascade ...IgG immunoglobulin complex / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / Role of phospholipids in phagocytosis / immunoglobulin complex, circulating / immunoglobulin receptor binding / FCGR3A-mediated IL10 synthesis / complement activation, classical pathway / Regulation of Complement cascade / FCGR3A-mediated phagocytosis / antigen binding / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / antibacterial humoral response / Interleukin-4 and Interleukin-13 signaling / adaptive immune response / blood microparticle / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Brady, R.L. / Hubbard, R.E. / Todd, R.J. | |||||||||
![]() | ![]() Title: Crystal structure of a chimeric Fab' fragment of an antibody binding tumour cells. Authors: Brady, R.L. / Edwards, D.J. / Hubbard, R.E. / Jiang, J.S. / Lange, G. / Roberts, S.M. / Todd, R.J. / Adair, J.R. / Emtage, J.S. / King, D.J. / Low, D.C. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Study of a Chimeric Fab' Fragment of an Antibody Binding Tumour Cells Authors: Brady, R.L. / Hubbard, R.E. / King, D.J. / Low, D.C. / Roberts, S.M. / Todd, R.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.3 KB | Display | ![]() |
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PDB format | ![]() | 139.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 397.8 KB | Display | ![]() |
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Full document | ![]() | 440.6 KB | Display | |
Data in XML | ![]() | 22.8 KB | Display | |
Data in CIF | ![]() | 33.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO L 8 2: ASN L 28 - ILE L 29 OMEGA =145.72 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 3: CIS PROLINE - PRO L 95 / 4: CIS PROLINE - PRO L 141 / 5: CIS PROLINE - PRO H 148 / 6: CIS PROLINE - PRO H 150 | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.999867, -0.01191, 0.011172), Vector: Details | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT CONTAINS TWO FAB MOLECULES. ONLY ONE MOLECULE IS PRESENTED IN THIS ENTRY. THE OTHER MOLECULE MAY BE GENERATED USING THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW. | |
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Components
#1: Antibody | Mass: 23152.539 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus, Homo sapiens / Production host: ![]() ![]() #2: Antibody | Mass: 22790.434 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus, Homo sapiens / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.57 Å3/Da / Density % sol: 65.57 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7 / Method: microdialysis | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 3.1 Å / Num. obs: 15644 / % possible obs: 74 % / Num. measured all: 57781 / Rmerge(I) obs: 0.068 |
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Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.176 / Highest resolution: 3.1 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 3.1 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 3.1 Å / Lowest resolution: 15 Å / Rfactor obs: 0.176 / Num. reflection obs: 15644 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 35.87 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 3.1 Å / Lowest resolution: 3.34 Å / Total num. of bins used: 5 / Rfactor obs: 0.2424 |