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Open data
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Basic information
Entry | Database: PDB / ID: 1bb6 | ||||||
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Title | LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE | ||||||
![]() | LYSOZYME | ||||||
![]() | HYDROLASE / N-ACETYL-MURAMIDASE / UMBELLIFERONE GLYCOSIDES | ||||||
Function / homology | ![]() lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Vollan, V.B. / Hough, E. / Karlsen, S. | ||||||
![]() | ![]() Title: Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme. Authors: Vollan, V.B. / Hough, E. / Karlsen, S. #1: ![]() Title: Crystal Structures of Three Complexes between Chito-Oligosaccharides and Lysozyme from the Rainbow Trout. How Distorted is the Nag Sugar in Site D? Authors: Karlsen, S. / Hough, E. #2: ![]() Title: Refined Crystal Structure of Lysozyme from the Rainbow Trout (Oncorhynchus Mykiss) Authors: Karlsen, S. / Eliassen, B.E. / Hansen, L.K. / Larsen, R.L. / Riise, B.W. / Smalas, A.O. / Hough, E. / Grinde, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 37.9 KB | Display | ![]() |
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PDB format | ![]() | 29.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 14303.068 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-UMG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.25 Å3/Da / Density % sol: 62 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 10 / Details: pH 10.0 | ||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 277 K / Method: vapor diffusion, hanging dropDetails: Karlsen, S., (1995) Acta Crystallogr.,Sect.D, 51, 354. PH range low: 10 / PH range high: 4 | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER / Date: Mar 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→28 Å / Num. obs: 11445 / % possible obs: 89.5 % / Observed criterion σ(I): 1 / Redundancy: 1.8 % / Rmerge(I) obs: 0.01 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 1.2 % / Mean I/σ(I) obs: 1.8 / % possible all: 42.7 |
Reflection | *PLUS Num. measured all: 20088 / Rmerge(I) obs: 0.105 |
Reflection shell | *PLUS % possible obs: 42.7 % |
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Processing
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Refinement | Method to determine structure: PHASES FOR NATIVE STRUCTURE / Resolution: 2→8 Å / Cross valid method: THROUGHOUT / σ(F): 1
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Displacement parameters | Biso mean: 18.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.211 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |