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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1bay | ||||||
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タイトル | GLUTATHIONE S-TRANSFERASE YFYF CYS 47-CARBOXYMETHYLATED CLASS PI, FREE ENZYME | ||||||
![]() | GLUTATHIONE S-TRANSFERASE CLASS PI | ||||||
![]() | TRANSFERASE / MULTIGENE FAMILY | ||||||
機能・相同性 | ![]() cellular response to cell-matrix adhesion / negative regulation of neutrophil aggregation / negative regulation of peroxidase activity / Paracetamol ADME / Glutathione conjugation / negative regulation of smooth muscle cell chemotaxis / Detoxification of Reactive Oxygen Species / kinase regulator activity / negative regulation of leukocyte proliferation / response to L-ascorbic acid ...cellular response to cell-matrix adhesion / negative regulation of neutrophil aggregation / negative regulation of peroxidase activity / Paracetamol ADME / Glutathione conjugation / negative regulation of smooth muscle cell chemotaxis / Detoxification of Reactive Oxygen Species / kinase regulator activity / negative regulation of leukocyte proliferation / response to L-ascorbic acid / common myeloid progenitor cell proliferation / organic cyclic compound binding / glutathione derivative biosynthetic process / hepoxilin biosynthetic process / negative regulation of nitric-oxide synthase biosynthetic process / oligodendrocyte development / negative regulation of JUN kinase activity / negative regulation of monocyte chemotactic protein-1 production / JUN kinase binding / cellular response to glucocorticoid stimulus / negative regulation of stress-activated MAPK cascade / negative regulation of interleukin-1 beta production / regulation of stress-activated MAPK cascade / prostaglandin metabolic process / negative regulation of vascular associated smooth muscle cell proliferation / negative regulation of acute inflammatory response / glutathione transferase / negative regulation of tumor necrosis factor production / glutathione transferase activity / toxic substance binding / response to amino acid / animal organ regeneration / negative regulation of fibroblast proliferation / negative regulation of canonical NF-kappaB signal transduction / cellular response to epidermal growth factor stimulus / Neutrophil degranulation / xenobiotic metabolic process / response to nutrient levels / glutathione metabolic process / regulation of ERK1 and ERK2 cascade / positive regulation of superoxide anion generation / negative regulation of MAP kinase activity / response to reactive oxygen species / negative regulation of extrinsic apoptotic signaling pathway / negative regulation of ERK1 and ERK2 cascade / response to toxic substance / cellular response to insulin stimulus / response to estradiol / cellular response to lipopolysaccharide / response to ethanol / negative regulation of apoptotic process / protein kinase binding / protein-containing complex / mitochondrion / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Vega, M.C. / Coll, M. | ||||||
![]() | ![]() タイトル: The three-dimensional structure of Cys-47-modified mouse liver glutathione S-transferase P1-1. Carboxymethylation dramatically decreases the affinity for glutathione and is associated ...タイトル: The three-dimensional structure of Cys-47-modified mouse liver glutathione S-transferase P1-1. Carboxymethylation dramatically decreases the affinity for glutathione and is associated with a loss of electron density in the alphaB-310B region. 著者: Vega, M.C. / Walsh, S.B. / Mantle, T.J. / Coll, M. #1: ![]() タイトル: Molecular Structure at 1.8 A of Mouse Liver Class Pi Glutathione S-Transferase Complexed with S-(P-Nitrobenzyl)Glutathione and Other Inhibitors 著者: Garcia-Saez, I. / Parraga, A. / Phillips, M.F. / Mantle, T.J. / Coll, M. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 89.7 KB | 表示 | ![]() |
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PDB形式 | ![]() | 69.1 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 419 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 421.1 KB | 表示 | |
XML形式データ | ![]() | 16.7 KB | 表示 | |
CIF形式データ | ![]() | 23.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 23503.965 Da / 分子数: 2 / 由来タイプ: 天然 詳細: CHEMICAL MODIFICATION ON CYS 47 WITH IODOACETIC ACID, CYS 47 CARBOXYMETHYLATED 由来: (天然) ![]() ![]() #2: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.95 Å3/Da / 溶媒含有率: 60.9 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | *PLUS pH: 7 / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射光源 | 由来: ![]() ![]() ![]() |
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検出器 | 検出器: IMAGE PLATE / 日付: 1995年7月22日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.937 Å / 相対比: 1 |
反射 | 解像度: 2→50 Å / Num. obs: 28194 / % possible obs: 75 % / Observed criterion σ(I): 4 / Rmerge(I) obs: 0.061 |
反射 シェル | 解像度: 2→2.5 Å / % possible all: 56.6 |
反射 | *PLUS Num. obs: 31316 / % possible obs: 78.7 % / Observed criterion σ(I): 3 |
反射 シェル | *PLUS 最低解像度: 2.2 Å / % possible obs: 56.8 % |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: MOLECULAR REEMPLACEMENT 開始モデル: PDB ENTRY 1GLQ 解像度: 2→8 Å / Data cutoff high absF: 50 / Data cutoff low absF: 2 / 交差検証法: BRUNGER'S METHOD / σ(F): 2
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精密化ステップ | サイクル: LAST / 解像度: 2→8 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.195 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |