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Yorodumi- PDB-1b4m: NMR STRUCTURE OF APO CELLULAR RETINOL-BINDING PROTEIN II, 24 STRU... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1b4m | ||||||
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Title | NMR STRUCTURE OF APO CELLULAR RETINOL-BINDING PROTEIN II, 24 STRUCTURES | ||||||
Components | CELLULAR RETINOL-BINDING PROTEIN II | ||||||
Keywords | RETINOL TRANSPORT / CELLULAR RETINOL-BINDING PROTEIN / LIPID TRANSPORT / CRBP II / LIPID-BINDING PROTEIN | ||||||
Function / homology | Function and homology information Retinoid metabolism and transport / retinal binding / retinol metabolic process / retinol binding / fatty acid transport / fatty acid binding / lipid binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / SIMULATED ANNEALING REFINEMENT | ||||||
Authors | Lu, J. / Lin, C.-L. / Tang, C. / Ponder, J.W. / Kao, J.L.F. / Cistola, D.P. / Li, E. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1999 Title: The structure and dynamics of rat apo-cellular retinol-binding protein II in solution: comparison with the X-ray structure. Authors: Lu, J. / Lin, C.L. / Tang, C. / Ponder, J.W. / Kao, J.L. / Cistola, D.P. / Li, E. #1: Journal: Biochemistry / Year: 1997 Title: Ligand Binding Alters the Backbone Mobility of Intestinal Fatty Acid-Binding Protein as Monitored by 15N NMR Relaxation and 1H Exchange Authors: Hodsdon, M.E. / Cistola, D.P. #2: Journal: J.Mol.Biol. / Year: 1996 Title: The NMR Solution Structure of Intestinal Fatty Acid-Binding Protein Complexed with Palmitate: Application of a Novel Distance Geometry Algorithm Authors: Hodsdon, M.E. / Ponder, J.W. / Cistola, D.P. #3: Journal: Adv.Protein Chem. / Year: 1994 Title: Lipid-Binding Proteins: A Family of Fatty Acid and Retinoid Transport Proteins Authors: Banaszak, L. / Winter, N. / Xu, Z. / Bernlohr, D.A. / Cowan, S. / Jones, T.A. #4: Journal: J.Mol.Biol. / Year: 1993 Title: Crystal Structures of Holo and Apo-Cellular Retinol-Binding Protein II Authors: Winter, N.S. / Bratt, J.M. / Banaszak, L.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b4m.cif.gz | 1004 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b4m.ent.gz | 873.2 KB | Display | PDB format |
PDBx/mmJSON format | 1b4m.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b4/1b4m ftp://data.pdbj.org/pub/pdb/validation_reports/b4/1b4m | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15606.570 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Cell: SMALL INTESTINAL ENTEROCYTE / Cellular location: CYTOPLASM / Plasmid: PMON-CRBP II / Production host: Escherichia coli (E. coli) / Strain (production host): JM101 / References: UniProt: P06768 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: THE LIMITS ON SECONDARY STRUCTURE ELEMENTS WERE DEFINED BY THE PROTON-CARBON CONSENSUS CHEMICAL SHIFT INDEX. |
-Sample preparation
Details | Contents: 90% WATER/10% D2O |
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Sample conditions | pH: 7.4 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANNEALING REFINEMENT / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | |||||||||
NMR ensemble | Conformer selection criteria: FINAL PENALTY FUNCTION VALUES WITHIN 2 STANDARD DEVIATIONS FROM THE MEAN Conformers calculated total number: 25 / Conformers submitted total number: 24 |