+Open data
-Basic information
Entry | Database: PDB / ID: 1b3p | ||||||||||||||||||
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Title | 5'-D(*GP*GP*AP*GP*GP*AP*T)-3' | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / (G-G-A) TRIPLET REPEAT / V-SHAPED BACKBONE / PARALLEL-STRANDED SEGMENTS / MISMATCH ALIGNMENTS / UNIFORM 13C / 15N-LABELED DNA | Function / homology | DNA | Function and homology information Method | SOLUTION NMR / distance geometry | Authors | Kettani, A. / Bouaziz, S. / Skripkin, E. / Majumdar, A. / Wang, W. / Jones, R.A. / Patel, D.J. | Citation | Journal: Structure Fold.Des. / Year: 1999 | Title: Interlocked mismatch-aligned arrowhead DNA motifs. Authors: Kettani, A. / Bouaziz, S. / Skripkin, E. / Majumdar, A. / Wang, W. / Jones, R.A. / Patel, D.J. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b3p.cif.gz | 151.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b3p.ent.gz | 122.6 KB | Display | PDB format |
PDBx/mmJSON format | 1b3p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b3p_validation.pdf.gz | 306.7 KB | Display | wwPDB validaton report |
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Full document | 1b3p_full_validation.pdf.gz | 393.1 KB | Display | |
Data in XML | 1b3p_validation.xml.gz | 9.8 KB | Display | |
Data in CIF | 1b3p_validation.cif.gz | 15.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b3/1b3p ftp://data.pdbj.org/pub/pdb/validation_reports/b3/1b3p | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 2202.472 Da / Num. of mol.: 2 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING 2D HOMONUCLEAR AND HETERONUCLEAR EXPERIMENTS ON 13C AND 15N LABELED SAMPLE. THE DISTANCE RESTRAINTS WERE DEDUCED FROM 5 NOESY MIXING TIMES (50, 100, 150, 200 ...Text: THE STRUCTURE WAS DETERMINED USING 2D HOMONUCLEAR AND HETERONUCLEAR EXPERIMENTS ON 13C AND 15N LABELED SAMPLE. THE DISTANCE RESTRAINTS WERE DEDUCED FROM 5 NOESY MIXING TIMES (50, 100, 150, 200 AND 250 MS) IN D2O AND 2 NOESY MIXING TIMES IN H2O (60 AND 200 MS) MS |
-Sample preparation
Sample conditions | Ionic strength: 10 mM NACL, 2 mM PHOSPHATE / pH: 6.6 / Pressure: ambient / Temperature: 273 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JNRL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: ACCEPTABLE COVALENT GEOMETRY, LOW DISTANCE RESTRAINTS VIIOLATIONS AND FAVORABLE NON-BONDED ENERGY VALUES Conformers calculated total number: 100 / Conformers submitted total number: 15 |