+Open data
-Basic information
Entry | Database: PDB / ID: 1aqi | ||||||
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Title | STRUCTURE OF ADENINE-N6-DNA-METHYLTRANSFERASE TAQI | ||||||
Components | ADENINE-N6-DNA-METHYLTRANSFERASE TAQI | ||||||
Keywords | METHYLTRANSFERASE / TRANSFERASE / RESTRICTION SYSTEM | ||||||
Function / homology | Function and homology information site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / DNA restriction-modification system / methylation / DNA binding Similarity search - Function | ||||||
Biological species | Thermus aquaticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Schluckebier, G. / Saenger, W. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M.TaqI. Authors: Schluckebier, G. / Kozak, M. / Bleimling, N. / Weinhold, E. / Saenger, W. #1: Journal: Gene / Year: 1995 Title: A Model for DNA Binding and Enzyme Action Derived from Crystallographic Studies of the TaqI N6-Adenine-Methyltransferase Authors: Schluckebier, G. / Labahn, J. / Granzin, J. / Schildkraut, I. / Saenger, W. #2: Journal: J.Mol.Biol. / Year: 1995 Title: Universal Catalytic Domain Structure of Adomet-Dependent Methyltransferases Authors: Schluckebier, G. / O'Gara, M. / Saenger, W. / Cheng, X. #3: Journal: Proc.Natl.Acad.Sci.USA / Year: 1994 Title: Three-Dimensional Structure of the Adenine-Specific DNA Methyltransferase M.Taq I in Complex with the Cofactor S-Adenosylmethionine Authors: Labahn, J. / Granzin, J. / Schluckebier, G. / Robinson, D.P. / Jack, W.E. / Schildkraut, I. / Saenger, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aqi.cif.gz | 165.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aqi.ent.gz | 130.6 KB | Display | PDB format |
PDBx/mmJSON format | 1aqi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1aqi_validation.pdf.gz | 996.8 KB | Display | wwPDB validaton report |
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Full document | 1aqi_full_validation.pdf.gz | 1015 KB | Display | |
Data in XML | 1aqi_validation.xml.gz | 30.3 KB | Display | |
Data in CIF | 1aqi_validation.cif.gz | 40.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/aq/1aqi ftp://data.pdbj.org/pub/pdb/validation_reports/aq/1aqi | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.99993, 0.0114, 0.00167), Vector: |
-Components
#1: Protein | Mass: 47931.191 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus aquaticus (bacteria) / Plasmid: PPR594 / Production host: Escherichia coli (E. coli) / Strain (production host): ER1821 References: UniProt: P14385, site-specific DNA-methyltransferase (adenine-specific) #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.58 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.3 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR571 / Wavelength: 1.5418 |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 1, 1994 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→10 Å / Num. obs: 30844 / % possible obs: 96 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Rmerge(I) obs: 0.066 |
Reflection | *PLUS Num. measured all: 110392 |
-Processing
Software |
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Refinement | Resolution: 2.6→10 Å / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 2.6→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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