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Yorodumi- PDB-1aoz: REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS R... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1aoz | ||||||
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Title | REFINED CRYSTAL STRUCTURE OF ASCORBATE OXIDASE AT 1.9 ANGSTROMS RESOLUTION | ||||||
Components | ASCORBATE OXIDASEL-ascorbate oxidase | ||||||
Keywords | OXIDOREDUCTASE(OXYGEN ACCEPTOR) | ||||||
Function / homology | Function and homology information L-ascorbate oxidase / L-ascorbate oxidase activity / copper ion binding / extracellular region Similarity search - Function | ||||||
Biological species | Cucurbita pepo var. melopepo (zucchini) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Messerschmidt, A. / Ladenstein, R. / Huber, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1992 Title: Refined crystal structure of ascorbate oxidase at 1.9 A resolution. Authors: Messerschmidt, A. / Ladenstein, R. / Huber, R. / Bolognesi, M. / Avigliano, L. / Petruzzelli, R. / Rossi, A. / Finazzi-Agro, A. #1: Journal: J.Mol.Biol. / Year: 1989 Title: X-Ray Crystal Structure of the Blue Oxidase Ascorbate Oxidase from Zucchini. Analysis of the Polypeptide Fold and a Model of the Copper Sites and Ligands Authors: Messerschmidt, A. / Rossi, A. / Ladenstein, R. / Huber, R. / Bolognesi, M. / Gatti, G. / Marchesini, A. / Petruzzelli, R. / Finazzi-Agro, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1aoz.cif.gz | 247.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1aoz.ent.gz | 199.2 KB | Display | PDB format |
PDBx/mmJSON format | 1aoz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ao/1aoz ftp://data.pdbj.org/pub/pdb/validation_reports/ao/1aoz | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 32 / 2: CIS PROLINE - PRO A 160 / 3: CIS PROLINE - PRO A 300 4: ASN A 551 - PRO A 552 OMEGA ANGLE = 88.478 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION 5: CIS PROLINE - PRO B 32 / 6: CIS PROLINE - PRO B 160 / 7: CIS PROLINE - PRO B 300 8: ASN B 551 - PRO B 552 OMEGA ANGLE = 76.915 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION | ||||||||||||
Components on special symmetry positions |
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-Components
-Protein / Sugars , 2 types, 4 molecules AB
#1: Protein | Mass: 61768.562 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cucurbita pepo var. melopepo (zucchini) Species: Cucurbita pepo / Strain: var. melopepo / References: UniProt: P37064, L-ascorbate oxidase #2: Sugar | |
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-Non-polymers , 4 types, 977 molecules
#3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.21 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / Method: microdialysisDetails: referred to 'Bolognesi, M.', (1983) J. Mol. Biol., 169, 351-352 PH range low: 5.8 / PH range high: 5.4 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 9999 Å / Num. obs: 86529 / % possible obs: 85.6 % / Observed criterion σ(I): 1 / Num. measured all: 249029 / Rmerge(I) obs: 0.094 |
-Processing
Software |
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Refinement | Highest resolution: 1.9 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.9 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 8 Å / Num. reflection obs: 85252 / Rfactor obs: 0.203 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 22.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.99 |